One-step immunoassay based on filtration for detection of food poisoning-related bacteria

Hong Rae Kim, Ji Hong Bong, Min Jung Kang, Won Bo Shim, Jeong Soo Lee, Jae Chul Pyun

Research output: Contribution to journalArticlepeer-review

Abstract

A one-step immunoassay based on filtration was presented, which used microbeads for target analyte detection and filters with appropriate pore sizes to distinguish the complexity of target analyte and microbeads. For effective bacterial detection, the microbead size and the filter's pore size must be optimized. The optimal concentrations of the enzyme (urease) and antibody were determined at the maximum absorbance change, that is, the maximum pH change. The pH change was measured using a field-effect transistor (FET). The correlation between pH change and threshold voltage was estimated to be 21.7 mV/pH, and the correlation between pH change and the source-drain current was estimated to be −379 nA/pH. For the one-step immunoassay, antibodies against target bacteria were isolated from horse serum by filtration, and these antibodies were estimated to have a sufficiently high specificity to overcome cross-reactivity among five types of food poisoning-related bacteria: Escherichia coli O157, Salmonella typhimurium, Listeria monocytogenes, Bacillus cereus, and Staphylococcus aureus. Finally, the FET-based one-step immunoassay was demonstrated for five types of food poisoning-related bacteria in human serum.

Original languageEnglish
Article number124203
JournalTalanta
Volume255
DOIs
Publication statusPublished - 2023 Apr 1

Bibliographical note

Funding Information:
The National Research Foundation of Korea (grant numbers: NRF-2020R1A2B5B01002187 , NRF-2020R1A5A101913111 , NRF-2021R1A2C209370611 , and NRF-2020R1A6A3A03040518 ) and the Korea Health Technology R&D Project of the Korea Health Industry Development Institute (KHIDI) supported this research (grant no: HI19C1344 ) .

Publisher Copyright:
© 2022 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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