A one-step immunoassay was developed for five types of food-poisoning-related bacteria using a switching peptide and antibodies isolated from unimmunized horse serum. The one-step immunoassay involves mixing samples and reagents in a homogeneous solution without any washing steps. In this work, a one-step immunoassay configuration was developed using isolated antibodies labelled with an organic fluorescence quencher and a switching-peptide labelled with a fluorescent dye. The fluorescence-labelled switching-peptide was bound to the antigen-binding site of the isolated antibodies before binding to the bacteria (no fluorescence signal), and the switching-peptide dissociated from the antibodies as soon as they bound to the bacteria (fluorescence signal turns on). By quantifying the generated fluorescence signal, the one-step immunoassay presented here allows microbial detection without any washing step.
Bibliographical noteFunding Information:
This work was supported by the National Research Foundation of Korea [grant numbers NRF-2020R1A2B5B01002187, 2020R1A5A101913111, and 2021R1A2C209370611].
© 2022 The Royal Society of Chemistry.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Environmental Chemistry