One-step PCR amplification of complete arthropod mitochondrial genomes

Ui Wook Hwang, Chan Jong Park, Taisoon Yong, Won Kim

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

A new PCR primer set which enables one-step amplification of complete arthropod mitochondrial genomes was designed from two conserved 16S rDNA regions for the long PCR technique. For this purpose, partial 16S rDNAs amplified with universal primers 16SA and 16SB were newly sequenced from six representative arthropods: Armadillidium vulgare and Macrobrachium nipponense (Crustacea), Anopheles sinensis (Insecta), Lithobius forficatus and Megaphyllum sp. (Myriapoda), and Limulus polyphemus (Chelicerata). The genomic locations of two new primers, HPK16Saa and HPK16Sbb, correspond to positions 13314-13345 and 12951-12984, respectively, in the Drosophila yakuba mitochondrial genome. The usefulness of the primer set was experimentally examined and confirmed with five of the representative arthropods, except for A. vulgare, which has a linearized mitochondrial genome. With this set, therefore, we could easily and rapidly amplify complete mitochondrial genomes with small amounts of arthropod DNA. Although the primers suggested here were examined only with arthropod groups, a possibility of successful application to other invertebrates is very high, since the high degree of sequence conservation is shown on the primer sites in other invertebrates. Thus, this primer set can serve various research fields, such as molecular evolution, population genetics, and molecular phylogenetics based on DNA sequences, RFLP, and gene rearrangement of mitochondrial genomes in arthropods and other invertebrates.

Original languageEnglish
Pages (from-to)345-352
Number of pages8
JournalMolecular Phylogenetics and Evolution
Volume19
Issue number3
DOIs
Publication statusPublished - 2001 Jan 1

Fingerprint

Mitochondrial Genome
Arthropods
arthropod
arthropods
amplification
genome
Polymerase Chain Reaction
Invertebrates
Armadillidium vulgare
invertebrate
invertebrates
Ribosomal DNA
Lithobius forficatus
Anopheles sinensis
Drosophila yakuba
Palaemonidae
Chelicerata
Myriapoda
Limulus polyphemus
Crustacea

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Hwang, Ui Wook ; Park, Chan Jong ; Yong, Taisoon ; Kim, Won. / One-step PCR amplification of complete arthropod mitochondrial genomes. In: Molecular Phylogenetics and Evolution. 2001 ; Vol. 19, No. 3. pp. 345-352.
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One-step PCR amplification of complete arthropod mitochondrial genomes. / Hwang, Ui Wook; Park, Chan Jong; Yong, Taisoon; Kim, Won.

In: Molecular Phylogenetics and Evolution, Vol. 19, No. 3, 01.01.2001, p. 345-352.

Research output: Contribution to journalArticle

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