Organic interfacial compounds (OICs) are required as linkers for the highly stable and efficient immobilization of bioprobes in nanobiosensors using 2D nanomaterials such as graphene. Herein, we first demonstrated the fabrication of a field-effect transistor (FET) via a microelectromechanical system process after covalent functionalization on large-scale graphene by introducing oligo(phenylene-ethynylene)amine (OPE). OPE was compared to various OICs by density functional theory simulations and was confirmed to have a higher binding energy with graphene and a lower band gap than other OICs. OPE can improve the immobilization efficiency of a bioprobe by forming a self-assembly monolayer via anion-based reaction. Using this technology, Magainin I-conjugated OGMFET (MOGMFET) showed a high sensitivity, high selectivity, with a limit of detection of 100 cfu mL−1. These results indicate that the OPE OIC can be applied for stable and comfortable interfacing technology for biosensor fabrication.
Bibliographical noteFunding Information:
This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) and Korea Smart Farm R&D Foundation (KosFarm) through Smart Farm Innovation Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) and Ministry of Science and ICT (MSIT), Rural Development Administration (RDA) (421020‐03); the National Research Council of Science & Technology (NST) grant by the Korea government (MSIT) (No. CAP22011‐000); Korea Ministry of Environment (MOE) through Technology Development Project for Safety Management of Household Chemical Products Program, funded by Korea Ministry of Environment (MOE) (2022002980005, 1485018893); National R&D Program of National Research Foundation of Korea (NRF) funded by Ministry of Science and ICT (NRF‐2021M3H4A4079381); Nanomedical Devices Development Project of NNFC; the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program (KGM2112234).
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