Optical transfection of mammalian cells

David Stevenson, Ben Agate, Lynn Paterson, Tanya Lake, Muriel Comrie, Tom Brown, Andrew Riches, Peter Bryant, Wilson Sibbett, Frank Gunn-Moore, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The introduction of naked DNA or other membrane impermeable substances into a cell (transfection) is a ubiquitous problem in cell biology. This problem is particularly challenging when it is desired to load membrane impermeable substances into specific cells, as most transfection technologies (such as liposomal transfection) are based on treating a global population of cells. The technique of optical transfection, using a focused laser to open a small transient hole in the membrane of a biological cell (photoporation) to load membrane impermeable DNA into it, allows individual cells to be targeted for transfection, while leaving neighbouring cells unaffected. Unlike other techniques used to perform single cell transfection, such as microinjection, optical transfection can be performed in an entirely closed system, thereby maintaining sterility of the sample during treatment. Here, we are investigating the introduction and subsequent expression of foreign DNA into living mammalian cells by laser-assisted photoporation with a femtosecond-pulsed titanium sapphire laser at 800 nm, in cells that are adherent.

Original languageEnglish
Title of host publicationBiophotonics and New Therapy Frontiers
DOIs
Publication statusPublished - 2006 Jun 22
EventBiophotonics and New Therapy Frontiers - Strasbourg, France
Duration: 2006 Apr 32006 Apr 5

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6191
ISSN (Print)1605-7422

Conference

ConferenceBiophotonics and New Therapy Frontiers
CountryFrance
CityStrasbourg
Period06/4/306/4/5

Fingerprint

Transfection
Cells
Membranes
DNA
cells
membranes
Lasers
deoxyribonucleic acid
Cytology
Aluminum Oxide
Titanium
Sapphire
lasers
sapphire
titanium
Microinjections
Infertility
Cell Biology
Technology
Population

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Stevenson, D., Agate, B., Paterson, L., Lake, T., Comrie, M., Brown, T., ... Dholakia, K. (2006). Optical transfection of mammalian cells. In Biophotonics and New Therapy Frontiers [61910D] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6191). https://doi.org/10.1117/12.662325
Stevenson, David ; Agate, Ben ; Paterson, Lynn ; Lake, Tanya ; Comrie, Muriel ; Brown, Tom ; Riches, Andrew ; Bryant, Peter ; Sibbett, Wilson ; Gunn-Moore, Frank ; Dholakia, Kishan. / Optical transfection of mammalian cells. Biophotonics and New Therapy Frontiers. 2006. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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Stevenson, D, Agate, B, Paterson, L, Lake, T, Comrie, M, Brown, T, Riches, A, Bryant, P, Sibbett, W, Gunn-Moore, F & Dholakia, K 2006, Optical transfection of mammalian cells. in Biophotonics and New Therapy Frontiers., 61910D, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6191, Biophotonics and New Therapy Frontiers, Strasbourg, France, 06/4/3. https://doi.org/10.1117/12.662325

Optical transfection of mammalian cells. / Stevenson, David; Agate, Ben; Paterson, Lynn; Lake, Tanya; Comrie, Muriel; Brown, Tom; Riches, Andrew; Bryant, Peter; Sibbett, Wilson; Gunn-Moore, Frank; Dholakia, Kishan.

Biophotonics and New Therapy Frontiers. 2006. 61910D (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6191).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Stevenson D, Agate B, Paterson L, Lake T, Comrie M, Brown T et al. Optical transfection of mammalian cells. In Biophotonics and New Therapy Frontiers. 2006. 61910D. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.662325