Optical transfection using an endoscope-like system

Nan Ma, Frank Gunn-Moore, Kishan Dholakia

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Optical transfection is a powerful method for targeted delivery of therapeutic agents to biological cells. A tightly focused pulsed laser beam may transiently change the permeability of a cell membrane to facilitate the delivery of foreign genetic material into cells. We report the first realization of an endoscope-like integrated system for optical transfection. An imaging fiber (coherent optical fiber bundle) with ∼6000 cores (pixels) embedded in a fiber cladding of ∼300 μm in diameter, produces an image circle (area) of ∼270 μm diam. This imaging fiber, with an ordered axicon lens array chemically etched at its exit face, is used for the delivery of a femtosecond laser to the cell membrane for optical transfection along with subcellular resolution imaging. A microcapillary-based microfluidic system for localized drug delivery was also combined in this miniature, flexible system. Using this novel system, a plasmid transfection efficiency up to ∼72% was obtained for CHO-K1 cells. This endoscope-like system opens a range of exciting applications, in particular, in the targeted in vivo optical microsurgery area.

Original languageEnglish
Article number028002
JournalJournal of Biomedical Optics
Volume16
Issue number2
DOIs
Publication statusPublished - 2011 Feb 1

Fingerprint

endoscopes
Endoscopy
delivery
Cell membranes
Imaging techniques
Fibers
fibers
Open systems
Ultrashort pulses
Pulsed lasers
Drug delivery
Microfluidics
plasmids
Laser beams
Optical fibers
Lenses
Plasmids
Pixels
cells
bundles

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

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Optical transfection using an endoscope-like system. / Ma, Nan; Gunn-Moore, Frank; Dholakia, Kishan.

In: Journal of Biomedical Optics, Vol. 16, No. 2, 028002, 01.02.2011.

Research output: Contribution to journalArticle

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