Optically guided neuronal growth at near-infrared wavelengths

D. J. Stevenson, T. K. Lake, B. Agate, V. Garcés-Chávez, K. Dholakia, F. Gunn-Moore

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Recent work has indicated the potential of light to guide the growth cones of neuronal cells using a Ti: Sapphire laser at 800 nm (Ehrlicher et al, PNAS, 2002). We have developed an optical set-up that has allowed, for the first time, the direct comparison of this process at near infrared wavelengths. A high number of growth cones were studied in order to provide a detailed statistical analysis. Actively extending growth cones of the neuroblastoma cell-line, NG108, can be guided at not only 780 nm, but also at 1064 nm. These wavelengths are an appropriate choice for guidance experiments, as wavelengths in the visible spectrum and UV are highly absorbing by cells and lead to death by phototoxicity and thermal stress. At 780 nm, 47% of actively extending growth cones were found to turn towards the focused incident light by at least 30° (n=32 growth cones). At 1064 nm, 61% of cells were successfully guided (n=31 growth cones). This suggests that the light detection mechanism within the cell is not due a single protein with a defined activity wavelength as occurs for example with the photoreceptor family of opsin proteins in the mammalian eye. We present two novel mechanisms of light induced neuronal guidance which are not related to temperature increases, or optical tweezing of the growth cone. We are also now identifying the signaling pathways that mediate this phenomenon.

Original languageEnglish
Title of host publicationOptical Trapping and Optical Micromanipulation III
DOIs
Publication statusPublished - 2006 Dec 1
EventOptical Trapping and Optical Micromanipulation III - San Diego, CA, United States
Duration: 2006 Aug 132006 Aug 17

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6326
ISSN (Print)0277-786X

Conference

ConferenceOptical Trapping and Optical Micromanipulation III
CountryUnited States
CitySan Diego, CA
Period06/8/1306/8/17

Fingerprint

Cones
cones
Infrared
Cone
Wavelength
Infrared radiation
wavelengths
Cell
cells
Guidance
Opsins
proteins
Proteins
Protein
photoreceptors
Signaling Pathways
Aluminum Oxide
Sapphire
Thermal Stress
thermal stresses

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Stevenson, D. J., Lake, T. K., Agate, B., Garcés-Chávez, V., Dholakia, K., & Gunn-Moore, F. (2006). Optically guided neuronal growth at near-infrared wavelengths. In Optical Trapping and Optical Micromanipulation III [63262B] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6326). https://doi.org/10.1117/12.680554
Stevenson, D. J. ; Lake, T. K. ; Agate, B. ; Garcés-Chávez, V. ; Dholakia, K. ; Gunn-Moore, F. / Optically guided neuronal growth at near-infrared wavelengths. Optical Trapping and Optical Micromanipulation III. 2006. (Proceedings of SPIE - The International Society for Optical Engineering).
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Stevenson, DJ, Lake, TK, Agate, B, Garcés-Chávez, V, Dholakia, K & Gunn-Moore, F 2006, Optically guided neuronal growth at near-infrared wavelengths. in Optical Trapping and Optical Micromanipulation III., 63262B, Proceedings of SPIE - The International Society for Optical Engineering, vol. 6326, Optical Trapping and Optical Micromanipulation III, San Diego, CA, United States, 06/8/13. https://doi.org/10.1117/12.680554

Optically guided neuronal growth at near-infrared wavelengths. / Stevenson, D. J.; Lake, T. K.; Agate, B.; Garcés-Chávez, V.; Dholakia, K.; Gunn-Moore, F.

Optical Trapping and Optical Micromanipulation III. 2006. 63262B (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6326).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Stevenson DJ, Lake TK, Agate B, Garcés-Chávez V, Dholakia K, Gunn-Moore F. Optically guided neuronal growth at near-infrared wavelengths. In Optical Trapping and Optical Micromanipulation III. 2006. 63262B. (Proceedings of SPIE - The International Society for Optical Engineering). https://doi.org/10.1117/12.680554