Optimal DNA structure of reverse-hairpin beacons for label-free and positive surface enhanced Raman scattering assays

Jisun Ki, Jinyoung Kim, Seeungmin Han, Eunji Jang, Taeksu Lee, Jung Sub Wi, Tae Geol Lee, Woonsung Na, Daesub Song, Seungjoo Haam

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We developed a label-free and positive-readout surface enhanced Raman scattering (SERS) assay using reverse-hairpin molecular beacons (RHBs) for the detection of RNA genetic markers associated with a high pathogenicity influenza (HPAI) virus. The structure of RHBs flexibly changed from a linear configuration (open state) to hairpin (closed state) upon targeting, such that the Raman label was closed on the SERS substrate and induced an increase of SERS intensity (OFF-to-ON). By improving sequence-specific RNA/DNA hybridization efficiency, we adjusted the stem-loop ratio of RHB, which was efficient at values of less than 1. The optimized RHBs exhibited dramatic changes in signal based on a fluorescence system in which the target was present. We demonstrated that the OFF-to-ON SERS system using RHB immobilized on silver-coated gold nanobowls permitted rapid hybridization. This proof-of-concept could provide a potential diagnostic tool for point-ofcare influenza virus detection.

Original languageEnglish
Pages (from-to)2352-2360
Number of pages9
JournalOptical Materials Express
Volume7
Issue number7
DOIs
Publication statusPublished - 2017

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials

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