Optimal DNA structure of reverse-hairpin beacons for label-free and positive surface enhanced Raman scattering assays

Jisun Ki, Jinyoung Kim, Seeungmin Han, Eunji Jang, Taeksu Lee, Jung Sub Wi, Tae Geol Lee, Woonsung Na, Daesub Song, Seungjoo Haam

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We developed a label-free and positive-readout surface enhanced Raman scattering (SERS) assay using reverse-hairpin molecular beacons (RHBs) for the detection of RNA genetic markers associated with a high pathogenicity influenza (HPAI) virus. The structure of RHBs flexibly changed from a linear configuration (open state) to hairpin (closed state) upon targeting, such that the Raman label was closed on the SERS substrate and induced an increase of SERS intensity (OFF-to-ON). By improving sequence-specific RNA/DNA hybridization efficiency, we adjusted the stem-loop ratio of RHB, which was efficient at values of less than 1. The optimized RHBs exhibited dramatic changes in signal based on a fluorescence system in which the target was present. We demonstrated that the OFF-to-ON SERS system using RHB immobilized on silver-coated gold nanobowls permitted rapid hybridization. This proof-of-concept could provide a potential diagnostic tool for point-ofcare influenza virus detection.

Original languageEnglish
Pages (from-to)2352-2360
Number of pages9
JournalOptical Materials Express
Issue number7
Publication statusPublished - 2017 Jan 1


All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials

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