Optimization of codon usage enhances the immunogenicity of a DNA vaccine encoding mycobacterial antigen Ag85B

Hyun Jeong Ko, Sung Youl Ko, Yeon Jeong Kim, Eun Gae Lee, Sang Nae Cho, Chang Yuil Kang

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

In spite of its many other benefits, DNA vaccine is limited in its application by its insufficient immunogenicity. One promising approach for enhancing its immunogenicity is to maximize its expression in the immunized host. In the current study, we investigated whether codon optimization of the mycobacterial antigen Ag85B gene could enhance the expression and immunogenicity of the Ag85B DNA vaccine. We generated a synthetic humanized Ag85B (HAg85B) gene in which codon usage was optimized for expression in human cells. DNA plasmids with codon-optimized HAg85B increased the level of protein expression in vitro and in vivo. DNA vaccine with hAg85B induced stronger Th1-like and cytotoxic T-cell immune responses in BALB/c mice and generated higher protective immunity in a BALB/c mouse model of Mycobacterium tuberculosis aerosol infection than did the DNA vaccine with wild-type Ag85B. Therefore, our results suggest that codon optimization of mycobacterial antigens (e.g., Ag85B) could improve protein expression and thereby enhance the immunogenicity of DNA vaccines against M. tuberculosis.

Original languageEnglish
Pages (from-to)5666-5674
Number of pages9
JournalInfection and Immunity
Volume73
Issue number9
DOIs
Publication statusPublished - 2005 Sep

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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