Optimization of dilute acid and enzymatic hydrolysis for dark fermentative hydrogen production from the empty fruit bunch of oil palm

Ralph Rolly Gonzales, Jun Seok Kim, Sang Hyoun Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Pretreatment of the empty fruit brunch (EFB) from oil palm was investigated for H 2 fermentation. The EFB was hydrolyzed at various temperatures, H 2 SO 4 concentrations, and reaction times. Subsequently, the acid-hydrolysate underwent enzymatic saccharification under various temperature, pH, and enzymatic loading conditions. Response surface methodology derived the optimum sugar concentration (SC), hydrogen production rate (HPR), and hydrogen yield (HY) as 28.30 g L −1 , 2601.24 mL H 2 L −1 d −1 , and 275.75 mL H 2 g −1 total sugar (TS), respectively, at 120 °C, 60 min of reaction, and 6 vol% H 2 SO 4 , with the combined severity factor of 1.75. Enzymatic hydrolysis enhanced the SC, HY, and HPR to 34.52 g L −1 , 283.91 mL H 2 g −1 TS, and 3266.86 mL H 2 L −1 d −1 , respectively, at 45 °C, pH 5.0, and 1.17 mg enzyme mL −1 . Dilute acid hydrolysis would be a viable pretreatment for biohydrogen production from EFB. Subsequent enzymatic hydrolysis can be performed if enhanced HPR is required.

Original languageEnglish
Pages (from-to)2191-2202
Number of pages12
JournalInternational Journal of Hydrogen Energy
DOIs
Publication statusPublished - 2019 Jan 22

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Palm oil
Enzymatic hydrolysis
fruits
hydrogen production
Hydrogen production
sugars
Fruits
Sugars
hydrolysis
oils
acids
optimization
Acids
pretreatment
Saccharification
Hydrogen
fermentation
hydrogen
reaction time
Fermentation

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

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title = "Optimization of dilute acid and enzymatic hydrolysis for dark fermentative hydrogen production from the empty fruit bunch of oil palm",
abstract = "Pretreatment of the empty fruit brunch (EFB) from oil palm was investigated for H 2 fermentation. The EFB was hydrolyzed at various temperatures, H 2 SO 4 concentrations, and reaction times. Subsequently, the acid-hydrolysate underwent enzymatic saccharification under various temperature, pH, and enzymatic loading conditions. Response surface methodology derived the optimum sugar concentration (SC), hydrogen production rate (HPR), and hydrogen yield (HY) as 28.30 g L −1 , 2601.24 mL H 2 L −1 d −1 , and 275.75 mL H 2 g −1 total sugar (TS), respectively, at 120 °C, 60 min of reaction, and 6 vol{\%} H 2 SO 4 , with the combined severity factor of 1.75. Enzymatic hydrolysis enhanced the SC, HY, and HPR to 34.52 g L −1 , 283.91 mL H 2 g −1 TS, and 3266.86 mL H 2 L −1 d −1 , respectively, at 45 °C, pH 5.0, and 1.17 mg enzyme mL −1 . Dilute acid hydrolysis would be a viable pretreatment for biohydrogen production from EFB. Subsequent enzymatic hydrolysis can be performed if enhanced HPR is required.",
author = "Gonzales, {Ralph Rolly} and Kim, {Jun Seok} and Kim, {Sang Hyoun}",
year = "2019",
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AU - Gonzales, Ralph Rolly

AU - Kim, Jun Seok

AU - Kim, Sang Hyoun

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N2 - Pretreatment of the empty fruit brunch (EFB) from oil palm was investigated for H 2 fermentation. The EFB was hydrolyzed at various temperatures, H 2 SO 4 concentrations, and reaction times. Subsequently, the acid-hydrolysate underwent enzymatic saccharification under various temperature, pH, and enzymatic loading conditions. Response surface methodology derived the optimum sugar concentration (SC), hydrogen production rate (HPR), and hydrogen yield (HY) as 28.30 g L −1 , 2601.24 mL H 2 L −1 d −1 , and 275.75 mL H 2 g −1 total sugar (TS), respectively, at 120 °C, 60 min of reaction, and 6 vol% H 2 SO 4 , with the combined severity factor of 1.75. Enzymatic hydrolysis enhanced the SC, HY, and HPR to 34.52 g L −1 , 283.91 mL H 2 g −1 TS, and 3266.86 mL H 2 L −1 d −1 , respectively, at 45 °C, pH 5.0, and 1.17 mg enzyme mL −1 . Dilute acid hydrolysis would be a viable pretreatment for biohydrogen production from EFB. Subsequent enzymatic hydrolysis can be performed if enhanced HPR is required.

AB - Pretreatment of the empty fruit brunch (EFB) from oil palm was investigated for H 2 fermentation. The EFB was hydrolyzed at various temperatures, H 2 SO 4 concentrations, and reaction times. Subsequently, the acid-hydrolysate underwent enzymatic saccharification under various temperature, pH, and enzymatic loading conditions. Response surface methodology derived the optimum sugar concentration (SC), hydrogen production rate (HPR), and hydrogen yield (HY) as 28.30 g L −1 , 2601.24 mL H 2 L −1 d −1 , and 275.75 mL H 2 g −1 total sugar (TS), respectively, at 120 °C, 60 min of reaction, and 6 vol% H 2 SO 4 , with the combined severity factor of 1.75. Enzymatic hydrolysis enhanced the SC, HY, and HPR to 34.52 g L −1 , 283.91 mL H 2 g −1 TS, and 3266.86 mL H 2 L −1 d −1 , respectively, at 45 °C, pH 5.0, and 1.17 mg enzyme mL −1 . Dilute acid hydrolysis would be a viable pretreatment for biohydrogen production from EFB. Subsequent enzymatic hydrolysis can be performed if enhanced HPR is required.

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