Optimization of energy transfer between quantum dots and gold nanoparticles in head-to-head configuration for detection of fusion gene

Taegyeong Kang, Hyun Chang Kim, Sang Woo Joo, So Yeong Lee, Ik Sung Ahn, Kyong Ah Yoon, Kangtaek Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We report simultaneous detection of the variants 3a and 3b in the oncogenic EML4-ALK fusion gene using cDNA from lung cancer patients based on energy transfer between CdSe/ZnS quantum dots (QDs) and gold nanoparticles (AuNPs). To overcome the limitations of the conventional detection methods based on energy transfer, we have applied a head-to-head configuration of QDs and AuNPs. This allowed us not only to use longer probe oligonucleotides than the conventional methods, but also to optimize interparticle distance between QDs and AuNPs for maximum sensitivity and selectivity. By functionalizing two types of QDs (i.e., green- and red-emitting QDs) and 5 nm AuNPs with probe oligonucleotides, we demonstrated that energy was selectively transferred from green-emitting QDs to AuNPs when variant 3a was present in target cDNA, or from red-emitting QDs to AuNPs with variant 3b, resulting in fluorescence quenching. Our method could successfully be used to simultaneously detect two variants of EML4-ALK fusion gene at the target DNA concentration (3.45 nM) that was lower than the previously reported limit of detection values based on energy transfer phenomenon.

Original languageEnglish
Pages (from-to)729-734
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume188
DOIs
Publication statusPublished - 2013 Sep 2

Fingerprint

Gold
genes
Energy transfer
Semiconductor quantum dots
Fusion reactions
Genes
fusion
energy transfer
quantum dots
gold
Nanoparticles
nanoparticles
optimization
configurations
Oligonucleotide Probes
oligonucleotides
Oligonucleotides
Complementary DNA
probes
lungs

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry
  • Electrical and Electronic Engineering

Cite this

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title = "Optimization of energy transfer between quantum dots and gold nanoparticles in head-to-head configuration for detection of fusion gene",
abstract = "We report simultaneous detection of the variants 3a and 3b in the oncogenic EML4-ALK fusion gene using cDNA from lung cancer patients based on energy transfer between CdSe/ZnS quantum dots (QDs) and gold nanoparticles (AuNPs). To overcome the limitations of the conventional detection methods based on energy transfer, we have applied a head-to-head configuration of QDs and AuNPs. This allowed us not only to use longer probe oligonucleotides than the conventional methods, but also to optimize interparticle distance between QDs and AuNPs for maximum sensitivity and selectivity. By functionalizing two types of QDs (i.e., green- and red-emitting QDs) and 5 nm AuNPs with probe oligonucleotides, we demonstrated that energy was selectively transferred from green-emitting QDs to AuNPs when variant 3a was present in target cDNA, or from red-emitting QDs to AuNPs with variant 3b, resulting in fluorescence quenching. Our method could successfully be used to simultaneously detect two variants of EML4-ALK fusion gene at the target DNA concentration (3.45 nM) that was lower than the previously reported limit of detection values based on energy transfer phenomenon.",
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Optimization of energy transfer between quantum dots and gold nanoparticles in head-to-head configuration for detection of fusion gene. / Kang, Taegyeong; Kim, Hyun Chang; Joo, Sang Woo; Lee, So Yeong; Ahn, Ik Sung; Yoon, Kyong Ah; Lee, Kangtaek.

In: Sensors and Actuators, B: Chemical, Vol. 188, 02.09.2013, p. 729-734.

Research output: Contribution to journalArticle

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AU - Yoon, Kyong Ah

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