Optimization of enterokinase fermentation using a recombinant Saccharomyces cerevisiae

Hyun Jung Kim, Young Hwan Kim, Young Hoon Roh, Baik L. Seong, Chul Soo Shin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The optimization of enterokinase fermentation was performed with a recombinant Saccharomyces cerevisiae. The maximum enterokinase yield and specific yield were obtained when the ratio of yeast extract to bacto peptone was 5:5. However, the fraction of plasmid-containing cells was approximately 65% for most nitrogen source ratios. From batch cultures, the minimum DOT level was found to be a key factor. Through DO-stat cultivations, the value was further optimized. As the minimum DOT level increased, the product yield and plasmid-containing cell fraction increased, whereas the ethanol concentration decreased. The optimum agitation speed and aeration rate in a 5 l jar were determined as 700 rpm and 2 vvm, respectively. Under these conditions, a high enterokinase yield of 3.8 mg/l was obtained with the final ethanol concentration of 0.4 g/l. The minimum DOT level was maintained to be 60% or more. Scale-up of enterokinase fermentation from a 5 l jar to a 300 l jar was successfully accomplished based on strategies to maintain a constant impeller tip speed and a minimum DOT level at 60% or more.

Original languageEnglish
Pages (from-to)717-722
Number of pages6
JournalProcess Biochemistry
Volume40
Issue number2
DOIs
Publication statusPublished - 2005 Feb 1

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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