High hydrostatic pressure (HHP) extraction method was optimized to maximize the extraction yield of kirenol from Siegesbeckia orientalis. Operating parameters such as extraction pressure (100-600 MPa), pressure holding time (3-20 min), feed-to-solvent ratio (1:10-1:90 (w/v)), and solvent (ethanol) concentration (0-100%) were investigated individually by mono-factor experiments. Then, the optimal extraction conditions were determined using response surface methodology. Box-Behnken design was applied to evaluate the effects of three independent variables (extraction pressure, solvent concentration, and feed-to-solvent ratio) on the extraction yield of kirenol from S. orientalis. Extraction pressure, solvent concentration, and feed-to-solvent ratio affected the extraction yields significantly, whereas the pressure holding time had no significant effect. The optimal processing conditions, which gave a maximum extraction yield of 85.9% kirenol from the raw material, were as follows: extracting pressure 320 MPa, pressure holding time 5min, ethanol concentration 18%, and feed-to-solvent ratio 1:76 (w/v).
All Science Journal Classification (ASJC) codes
- Food Science
- Applied Microbiology and Biotechnology