Optofluidic twin-core hollow fiber interferometer for label-free sensing of the streptavidin-biotin binding

Xinghua Yang, Wenting Yu, Zhihai Liu, Jun Yang, Yu Zhang, Depeng Kong, Qunlong Long, Tingting Yuan, Jiaming Cao, Libo Yuan, Kyunghwan Oh

Research output: Contribution to journalArticle

Abstract

We proposed and experimentally demonstrated an optofluidic biosensor using a twin-core hollow optical fiber (TC-HOF) interferometer for label-free sensing of the streptavidin-biotin binding. The TC-HOF had a central hole and two cores were imbedded near its circumference. The central hole functioned as a microfluidic channel where the liquid was flown. One of the cores was suspended at the inner surface of the hole and served as sensing arm while the other core embedded in the cladding as the reference arm, which formed an integrated all-fiber Mach-Zehnder interferometer (MZI). Both ends of TC-HOF were serially spliced to multimode fiber-single mode fiber assembly, whose lateral positions were adjusted to maximize the interference visibility. Before the surface functionalization, the pristine TC-HOF MZI showed the RI sensitivity of 2577 nm/RIU. The suspended core's surface was chemically modified with streptavidin and biotin solutions were injected through micro-HOles in TC-HOF. Through the specific binding reaction between biotin and streptavidin, the effective refractive index (RI) around the sensing arm was modulated, which was detected by the spectral shifts in the interference patterns. The proposed sensor showed an excellent linear response to the biotin concentration in the range of 0.01–0.1 mg/mL and the net detection sensitivity of biotin was 16.9 nm/(mg/mL), which was achieved in a label-free manner. This device can be further applied to sensing and monitoring of the biochemical binding of biotin with various biomolecules such as cells, antigens, antibodies, and DNA in real-time and in situ.

Original languageEnglish
Pages (from-to)353-359
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume277
DOIs
Publication statusPublished - 2018 Dec 20

Fingerprint

biotin
Streptavidin
Biotin
Interferometers
Labels
hollow
interferometers
Optical fibers
fibers
Fibers
optical fibers
Mach-Zehnder interferometers
Refractive index
Multimode fibers
Biomolecules
Single mode fibers
Antigens
Microfluidics
Biosensors
Antibodies

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Yang, Xinghua ; Yu, Wenting ; Liu, Zhihai ; Yang, Jun ; Zhang, Yu ; Kong, Depeng ; Long, Qunlong ; Yuan, Tingting ; Cao, Jiaming ; Yuan, Libo ; Oh, Kyunghwan. / Optofluidic twin-core hollow fiber interferometer for label-free sensing of the streptavidin-biotin binding. In: Sensors and Actuators, B: Chemical. 2018 ; Vol. 277. pp. 353-359.
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abstract = "We proposed and experimentally demonstrated an optofluidic biosensor using a twin-core hollow optical fiber (TC-HOF) interferometer for label-free sensing of the streptavidin-biotin binding. The TC-HOF had a central hole and two cores were imbedded near its circumference. The central hole functioned as a microfluidic channel where the liquid was flown. One of the cores was suspended at the inner surface of the hole and served as sensing arm while the other core embedded in the cladding as the reference arm, which formed an integrated all-fiber Mach-Zehnder interferometer (MZI). Both ends of TC-HOF were serially spliced to multimode fiber-single mode fiber assembly, whose lateral positions were adjusted to maximize the interference visibility. Before the surface functionalization, the pristine TC-HOF MZI showed the RI sensitivity of 2577 nm/RIU. The suspended core's surface was chemically modified with streptavidin and biotin solutions were injected through micro-HOles in TC-HOF. Through the specific binding reaction between biotin and streptavidin, the effective refractive index (RI) around the sensing arm was modulated, which was detected by the spectral shifts in the interference patterns. The proposed sensor showed an excellent linear response to the biotin concentration in the range of 0.01–0.1 mg/mL and the net detection sensitivity of biotin was 16.9 nm/(mg/mL), which was achieved in a label-free manner. This device can be further applied to sensing and monitoring of the biochemical binding of biotin with various biomolecules such as cells, antigens, antibodies, and DNA in real-time and in situ.",
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Optofluidic twin-core hollow fiber interferometer for label-free sensing of the streptavidin-biotin binding. / Yang, Xinghua; Yu, Wenting; Liu, Zhihai; Yang, Jun; Zhang, Yu; Kong, Depeng; Long, Qunlong; Yuan, Tingting; Cao, Jiaming; Yuan, Libo; Oh, Kyunghwan.

In: Sensors and Actuators, B: Chemical, Vol. 277, 20.12.2018, p. 353-359.

Research output: Contribution to journalArticle

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AU - Yang, Xinghua

AU - Yu, Wenting

AU - Liu, Zhihai

AU - Yang, Jun

AU - Zhang, Yu

AU - Kong, Depeng

AU - Long, Qunlong

AU - Yuan, Tingting

AU - Cao, Jiaming

AU - Yuan, Libo

AU - Oh, Kyunghwan

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N2 - We proposed and experimentally demonstrated an optofluidic biosensor using a twin-core hollow optical fiber (TC-HOF) interferometer for label-free sensing of the streptavidin-biotin binding. The TC-HOF had a central hole and two cores were imbedded near its circumference. The central hole functioned as a microfluidic channel where the liquid was flown. One of the cores was suspended at the inner surface of the hole and served as sensing arm while the other core embedded in the cladding as the reference arm, which formed an integrated all-fiber Mach-Zehnder interferometer (MZI). Both ends of TC-HOF were serially spliced to multimode fiber-single mode fiber assembly, whose lateral positions were adjusted to maximize the interference visibility. Before the surface functionalization, the pristine TC-HOF MZI showed the RI sensitivity of 2577 nm/RIU. The suspended core's surface was chemically modified with streptavidin and biotin solutions were injected through micro-HOles in TC-HOF. Through the specific binding reaction between biotin and streptavidin, the effective refractive index (RI) around the sensing arm was modulated, which was detected by the spectral shifts in the interference patterns. The proposed sensor showed an excellent linear response to the biotin concentration in the range of 0.01–0.1 mg/mL and the net detection sensitivity of biotin was 16.9 nm/(mg/mL), which was achieved in a label-free manner. This device can be further applied to sensing and monitoring of the biochemical binding of biotin with various biomolecules such as cells, antigens, antibodies, and DNA in real-time and in situ.

AB - We proposed and experimentally demonstrated an optofluidic biosensor using a twin-core hollow optical fiber (TC-HOF) interferometer for label-free sensing of the streptavidin-biotin binding. The TC-HOF had a central hole and two cores were imbedded near its circumference. The central hole functioned as a microfluidic channel where the liquid was flown. One of the cores was suspended at the inner surface of the hole and served as sensing arm while the other core embedded in the cladding as the reference arm, which formed an integrated all-fiber Mach-Zehnder interferometer (MZI). Both ends of TC-HOF were serially spliced to multimode fiber-single mode fiber assembly, whose lateral positions were adjusted to maximize the interference visibility. Before the surface functionalization, the pristine TC-HOF MZI showed the RI sensitivity of 2577 nm/RIU. The suspended core's surface was chemically modified with streptavidin and biotin solutions were injected through micro-HOles in TC-HOF. Through the specific binding reaction between biotin and streptavidin, the effective refractive index (RI) around the sensing arm was modulated, which was detected by the spectral shifts in the interference patterns. The proposed sensor showed an excellent linear response to the biotin concentration in the range of 0.01–0.1 mg/mL and the net detection sensitivity of biotin was 16.9 nm/(mg/mL), which was achieved in a label-free manner. This device can be further applied to sensing and monitoring of the biochemical binding of biotin with various biomolecules such as cells, antigens, antibodies, and DNA in real-time and in situ.

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