Optogenetic neuronal stimulation promotes axon outgrowth and myelination of motor neurons in a three-dimensional motor neuron–Schwann cell coculture model on a microfluidic biochip

Sujin Hyung, Seung Ryeol Lee, Yeon Jee Kim, Seokyoung Bang, Dongha Tahk, Jong Chul Park, Jun Kyo Francis Suh, Noo Li Jeon

Research output: Contribution to journalArticle

Abstract

Axonal regeneration and remyelination of peripheral motor neurons (MNs) are critical for restoring neuromuscular motor function after injury or peripheral neuropathy. We examined whether optogenetically mediated light stimulation (OMLS) could enhance the axon outgrowth and myelination of MNs using three-dimensional motor neuron–Schwann cell (MN–SC) coculture on a microfluidic biochip. The biochip was designed to allow SCs to interact with the axons of MNs, while preventing direct contact between SCs and the cell bodies of MNs. Following coculture with SCs on the microfluidic biochip, MNs were transfected with a light-sensitive channelrhodopsin gene. Transfected MNs subjected to repeated light stimulation (20 Hz, 1 hr) produced significantly longer axons than nontransfected MNs. OMLS of MNs greatly increased the number of myelin basic protein (MBP)-expressing SCs, promoting the initiation of myelination of MNs. Ultrastructurally, OMLS of MNs markedly enhanced the thickness of the compact myelin sheath around the MN axons such that the average thickness was closer to that of the theoretical estimates in vivo. Thus, the MN–SC coculture model on a microfluidic biochip augmented by OMLS of MNs is a feasible platform for studying the relationship of neuronal activity with regrowth and remyelination.

Original languageEnglish
Pages (from-to)2425-2438
Number of pages14
JournalBiotechnology and Bioengineering
Volume116
Issue number10
DOIs
Publication statusPublished - 2019 Oct 1

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Optogenetics
Biochips
Microfluidics
Motor Neurons
Coculture Techniques
Neurons
Light
Axons
Neuronal Outgrowth
Myelin Basic Protein
Peripheral Nervous System Diseases
Myelin Sheath

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Hyung, Sujin ; Lee, Seung Ryeol ; Kim, Yeon Jee ; Bang, Seokyoung ; Tahk, Dongha ; Park, Jong Chul ; Suh, Jun Kyo Francis ; Jeon, Noo Li. / Optogenetic neuronal stimulation promotes axon outgrowth and myelination of motor neurons in a three-dimensional motor neuron–Schwann cell coculture model on a microfluidic biochip. In: Biotechnology and Bioengineering. 2019 ; Vol. 116, No. 10. pp. 2425-2438.
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Optogenetic neuronal stimulation promotes axon outgrowth and myelination of motor neurons in a three-dimensional motor neuron–Schwann cell coculture model on a microfluidic biochip. / Hyung, Sujin; Lee, Seung Ryeol; Kim, Yeon Jee; Bang, Seokyoung; Tahk, Dongha; Park, Jong Chul; Suh, Jun Kyo Francis; Jeon, Noo Li.

In: Biotechnology and Bioengineering, Vol. 116, No. 10, 01.10.2019, p. 2425-2438.

Research output: Contribution to journalArticle

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