Optogenetic stimulation promotes Schwann cell proliferation, differentiation, and myelination in vitro

Kyuhwan Jung, Ji Hye Park, Sung Yon Kim, Noo Li Jeon, Sung Rae Cho, Sujin Hyung

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Schwann cells (SCs) constitute a crucial element of the peripheral nervous system, by structurally supporting the formation of myelin and conveying vital trophic factors to the nervous system. However, the functions of SCs in developmental and regenerative stages remain unclear. Here, we investigated how optogenetic stimulation (OS) of SCs regulates their development. In SC monoculture, OS substantially enhanced SC proliferation and the number of BrdU + -S100ß + -SCs over time. In addition, OS also markedly promoted the expression of both Krox20 and myelin basic protein (MBP) in SC culture medium containing dBcAMP/NRG1, which induced differentiation. We found that the effects of OS are dependent on the intracellular Ca 2+ level. OS induces elevated intracellular Ca 2+ levels through the T-type voltage-gated calcium channel (VGCC) and mobilization of Ca 2+ from both inositol 1,4,5-trisphosphate (IP 3 )-sensitive stores and caffeine/ryanodine-sensitive stores. Furthermore, we confirmed that OS significantly increased expression levels of both Krox20 and MBP in SC-motor neuron (MN) coculture, which was notably prevented by pharmacological intervention with Ca 2+ . Taken together, our results demonstrate that OS of SCs increases the intracellular Ca 2+ level and can regulate proliferation, differentiation, and myelination, suggesting that OS of SCs may offer a new approach to the treatment of neurodegenerative disorders.

Original languageEnglish
Article number3487
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

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Optogenetics
Schwann Cells
Cell Differentiation
Cell Proliferation
Myelin Basic Protein
In Vitro Techniques
Ryanodine
Inositol 1,4,5-Trisphosphate
Peripheral Nervous System
Motor Neurons
Bromodeoxyuridine
Calcium Channels
Myelin Sheath
Coculture Techniques
Caffeine
Neurodegenerative Diseases
Nervous System
Culture Media
Cell Culture Techniques
Cell Count

All Science Journal Classification (ASJC) codes

  • General

Cite this

Jung, Kyuhwan ; Park, Ji Hye ; Kim, Sung Yon ; Jeon, Noo Li ; Cho, Sung Rae ; Hyung, Sujin. / Optogenetic stimulation promotes Schwann cell proliferation, differentiation, and myelination in vitro. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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abstract = "Schwann cells (SCs) constitute a crucial element of the peripheral nervous system, by structurally supporting the formation of myelin and conveying vital trophic factors to the nervous system. However, the functions of SCs in developmental and regenerative stages remain unclear. Here, we investigated how optogenetic stimulation (OS) of SCs regulates their development. In SC monoculture, OS substantially enhanced SC proliferation and the number of BrdU + -S100{\ss} + -SCs over time. In addition, OS also markedly promoted the expression of both Krox20 and myelin basic protein (MBP) in SC culture medium containing dBcAMP/NRG1, which induced differentiation. We found that the effects of OS are dependent on the intracellular Ca 2+ level. OS induces elevated intracellular Ca 2+ levels through the T-type voltage-gated calcium channel (VGCC) and mobilization of Ca 2+ from both inositol 1,4,5-trisphosphate (IP 3 )-sensitive stores and caffeine/ryanodine-sensitive stores. Furthermore, we confirmed that OS significantly increased expression levels of both Krox20 and MBP in SC-motor neuron (MN) coculture, which was notably prevented by pharmacological intervention with Ca 2+ . Taken together, our results demonstrate that OS of SCs increases the intracellular Ca 2+ level and can regulate proliferation, differentiation, and myelination, suggesting that OS of SCs may offer a new approach to the treatment of neurodegenerative disorders.",
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Optogenetic stimulation promotes Schwann cell proliferation, differentiation, and myelination in vitro. / Jung, Kyuhwan; Park, Ji Hye; Kim, Sung Yon; Jeon, Noo Li; Cho, Sung Rae; Hyung, Sujin.

In: Scientific reports, Vol. 9, No. 1, 3487, 01.12.2019.

Research output: Contribution to journalArticle

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AU - Hyung, Sujin

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