Orai1 expression is closely related with favorable prognostic factors in clear cell renal cell carcinoma

Sayamaa Lkhagvadorj, Ji Hee Kim, Sung Soo Oh, Mi Ra Lee, Jaehung Jung, Hyunchul Chung, Seungkuy Cha, Minseob Eom

Research output: Contribution to journalArticle

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Abstract

Store-operated calcium (Ca 2+ ) entry (SOCE) is the principal Ca 2+ entry route in non-excitable cells, including cancer cells. We previously demonstrated that Orai1 and STIM1, the molecular components of SOCE, are involved in tumorigenesis of clear cell renal cell carcinoma (CCRCC). However, a clinical relevance of Orai1 and STIM1 expression in CCRCC has been ill-defined. Here, we investigated the expression of Orai1 and STIM1 in CCRCC, and compared their expression with clinico-pathological parameters of CCRCC and the patients' outcome. Immunohistochemical staining for Orai1 and STIM1 was performed on 126 formalin fixed paraffin embedded tissue of CCRCC and western blot analysis for Orai1 was performed on the available fresh tissue. The results were compared with generally well-established clinicopathologic prognostic factors in CCRCC and patient survival. Membrane protein Orai1 is expressed in the nuclei in CCRCC, whereas STIM1 shows the cytosolic expression pattern in immunohistochemical staining. Orai1 expression level is inversely correlated with CCRCC tumor grade, whereas STIM1 expression level is not associated with tumor grade. The higher Orai1 expression is significantly associated with lower Fuhrman nuclear grade, pathologic T stage, and TNM stage and with favorable prognosis. The expression level of STIM1 is not correlated with CCRCC grade and clinical outcomes. Orai1 expression in CCRCC is associated with tumor progression and with favorable prognostic factors. These results suggest that Orai1 is an attractive prognostic marker and therapeutic target for CCRCC.

Original languageEnglish
Pages (from-to)879-885
Number of pages7
JournalJournal of Korean medical science
Volume31
Issue number6
DOIs
Publication statusPublished - 2016 Jun 1

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Renal Cell Carcinoma
Neoplasms
Staining and Labeling
Paraffin
Formaldehyde
Membrane Proteins
Carcinogenesis
Western Blotting
Calcium
Survival

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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title = "Orai1 expression is closely related with favorable prognostic factors in clear cell renal cell carcinoma",
abstract = "Store-operated calcium (Ca 2+ ) entry (SOCE) is the principal Ca 2+ entry route in non-excitable cells, including cancer cells. We previously demonstrated that Orai1 and STIM1, the molecular components of SOCE, are involved in tumorigenesis of clear cell renal cell carcinoma (CCRCC). However, a clinical relevance of Orai1 and STIM1 expression in CCRCC has been ill-defined. Here, we investigated the expression of Orai1 and STIM1 in CCRCC, and compared their expression with clinico-pathological parameters of CCRCC and the patients' outcome. Immunohistochemical staining for Orai1 and STIM1 was performed on 126 formalin fixed paraffin embedded tissue of CCRCC and western blot analysis for Orai1 was performed on the available fresh tissue. The results were compared with generally well-established clinicopathologic prognostic factors in CCRCC and patient survival. Membrane protein Orai1 is expressed in the nuclei in CCRCC, whereas STIM1 shows the cytosolic expression pattern in immunohistochemical staining. Orai1 expression level is inversely correlated with CCRCC tumor grade, whereas STIM1 expression level is not associated with tumor grade. The higher Orai1 expression is significantly associated with lower Fuhrman nuclear grade, pathologic T stage, and TNM stage and with favorable prognosis. The expression level of STIM1 is not correlated with CCRCC grade and clinical outcomes. Orai1 expression in CCRCC is associated with tumor progression and with favorable prognostic factors. These results suggest that Orai1 is an attractive prognostic marker and therapeutic target for CCRCC.",
author = "Sayamaa Lkhagvadorj and Kim, {Ji Hee} and Oh, {Sung Soo} and Lee, {Mi Ra} and Jaehung Jung and Hyunchul Chung and Seungkuy Cha and Minseob Eom",
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Orai1 expression is closely related with favorable prognostic factors in clear cell renal cell carcinoma. / Lkhagvadorj, Sayamaa; Kim, Ji Hee; Oh, Sung Soo; Lee, Mi Ra; Jung, Jaehung; Chung, Hyunchul; Cha, Seungkuy; Eom, Minseob.

In: Journal of Korean medical science, Vol. 31, No. 6, 01.06.2016, p. 879-885.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Orai1 expression is closely related with favorable prognostic factors in clear cell renal cell carcinoma

AU - Lkhagvadorj, Sayamaa

AU - Kim, Ji Hee

AU - Oh, Sung Soo

AU - Lee, Mi Ra

AU - Jung, Jaehung

AU - Chung, Hyunchul

AU - Cha, Seungkuy

AU - Eom, Minseob

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Store-operated calcium (Ca 2+ ) entry (SOCE) is the principal Ca 2+ entry route in non-excitable cells, including cancer cells. We previously demonstrated that Orai1 and STIM1, the molecular components of SOCE, are involved in tumorigenesis of clear cell renal cell carcinoma (CCRCC). However, a clinical relevance of Orai1 and STIM1 expression in CCRCC has been ill-defined. Here, we investigated the expression of Orai1 and STIM1 in CCRCC, and compared their expression with clinico-pathological parameters of CCRCC and the patients' outcome. Immunohistochemical staining for Orai1 and STIM1 was performed on 126 formalin fixed paraffin embedded tissue of CCRCC and western blot analysis for Orai1 was performed on the available fresh tissue. The results were compared with generally well-established clinicopathologic prognostic factors in CCRCC and patient survival. Membrane protein Orai1 is expressed in the nuclei in CCRCC, whereas STIM1 shows the cytosolic expression pattern in immunohistochemical staining. Orai1 expression level is inversely correlated with CCRCC tumor grade, whereas STIM1 expression level is not associated with tumor grade. The higher Orai1 expression is significantly associated with lower Fuhrman nuclear grade, pathologic T stage, and TNM stage and with favorable prognosis. The expression level of STIM1 is not correlated with CCRCC grade and clinical outcomes. Orai1 expression in CCRCC is associated with tumor progression and with favorable prognostic factors. These results suggest that Orai1 is an attractive prognostic marker and therapeutic target for CCRCC.

AB - Store-operated calcium (Ca 2+ ) entry (SOCE) is the principal Ca 2+ entry route in non-excitable cells, including cancer cells. We previously demonstrated that Orai1 and STIM1, the molecular components of SOCE, are involved in tumorigenesis of clear cell renal cell carcinoma (CCRCC). However, a clinical relevance of Orai1 and STIM1 expression in CCRCC has been ill-defined. Here, we investigated the expression of Orai1 and STIM1 in CCRCC, and compared their expression with clinico-pathological parameters of CCRCC and the patients' outcome. Immunohistochemical staining for Orai1 and STIM1 was performed on 126 formalin fixed paraffin embedded tissue of CCRCC and western blot analysis for Orai1 was performed on the available fresh tissue. The results were compared with generally well-established clinicopathologic prognostic factors in CCRCC and patient survival. Membrane protein Orai1 is expressed in the nuclei in CCRCC, whereas STIM1 shows the cytosolic expression pattern in immunohistochemical staining. Orai1 expression level is inversely correlated with CCRCC tumor grade, whereas STIM1 expression level is not associated with tumor grade. The higher Orai1 expression is significantly associated with lower Fuhrman nuclear grade, pathologic T stage, and TNM stage and with favorable prognosis. The expression level of STIM1 is not correlated with CCRCC grade and clinical outcomes. Orai1 expression in CCRCC is associated with tumor progression and with favorable prognostic factors. These results suggest that Orai1 is an attractive prognostic marker and therapeutic target for CCRCC.

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JF - Journal of Korean Medical Science

SN - 1011-8934

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