Origami-paper-based device for microvesicle/exosome preconcentration and isolation

Hyerin Kim; Kyu Hyoung Lee; Sung Il Han; Dongho Lee; Seok Chung; Dohwan Lee; Jeong Hoon Lee

doi:10.1039/c9lc00796b

Origami-paper-based device for microvesicle/exosome preconcentration and isolation

Hyerin Kim, Kyu Hyoung Lee, Sung Il Han, Dongho Lee, Seok Chung, Dohwan Lee, Jeong Hoon Lee

Department of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Microvesicles and exosomes are promising liquid biopsy biomarkers. However, conventional isolation techniques damage and contaminate the biomarkers. We developed an origami-paper-based device for effective isolation of biomarkers with less damage and in fewer steps. The multi-folded device enables the preconcentration of the microvesicles/exosomes on specific layers (∼5-fold) by the ion concentration polarization technique and they were simply isolated from the rest of the sample by unfolding the device.

Original language	English
Pages (from-to)	3917-3921
Number of pages	5
Journal	Lab on a chip
Volume	19
Issue number	23
DOIs	https://doi.org/10.1039/c9lc00796b
Publication status	Published - 2019 Dec 7

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation of Korea, Grant NRF-2018R1D1A1A09084044. J. H. Lee was supported by a research grant from Kwangwoon University in 2019. Hyerin Kim was supported by the “Competency Development Program for Industry Specialists” of the Korean Ministry of Trade, Industry and Energy (MOTIE), operated by the Korea Institute for Advancement of Technology (KIAT) (No. P0002397, HRD program for Industrial Convergence of Wearable Smart Devices).

All Science Journal Classification (ASJC) codes

Bioengineering
Biochemistry
Chemistry(all)
Biomedical Engineering

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10.1039/c9lc00796b

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abstract = "Microvesicles and exosomes are promising liquid biopsy biomarkers. However, conventional isolation techniques damage and contaminate the biomarkers. We developed an origami-paper-based device for effective isolation of biomarkers with less damage and in fewer steps. The multi-folded device enables the preconcentration of the microvesicles/exosomes on specific layers (∼5-fold) by the ion concentration polarization technique and they were simply isolated from the rest of the sample by unfolding the device.",

author = "Hyerin Kim and Lee, {Kyu Hyoung} and Han, {Sung Il} and Dongho Lee and Seok Chung and Dohwan Lee and Lee, {Jeong Hoon}",

note = "Funding Information: This study was supported by the National Research Foundation of Korea, Grant NRF-2018R1D1A1A09084044. J. H. Lee was supported by a research grant from Kwangwoon University in 2019. Hyerin Kim was supported by the “Competency Development Program for Industry Specialists” of the Korean Ministry of Trade, Industry and Energy (MOTIE), operated by the Korea Institute for Advancement of Technology (KIAT) (No. P0002397, HRD program for Industrial Convergence of Wearable Smart Devices).",

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AU - Lee, Dongho

AU - Chung, Seok

AU - Lee, Dohwan

AU - Lee, Jeong Hoon

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