OsATL38 mediates mono-ubiquitination of the 14-3-3 protein OsGF14d and negatively regulates the cold stress response in rice

Li Hua Cui, Hye Jo Min, Seong Guan Yu, Mi Young Byun, Tae Rin Oh, Andosung Lee, Hee Woong Yang, Woo Taek Kim

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


One of the major regulatory pathways that permits plants to convert an external stimulus into an internal cellular response within a short period of time is the ubiquitination pathway. In this study, OsATL38 was identified as a low temperature-induced gene that encodes a rice homolog of Arabidopsis Tóxicos en Levadura RING-type E3 ubiquitin (Ub) ligase, which was predominantly localized to the plasma membrane. OsATL38-overexpressing transgenic rice plants exhibited decreased tolerance to cold stress as compared with wild-type rice plants. In contrast, RNAi-mediated OsATL38 knockdown transgenic progeny exhibited markedly increased tolerance to cold stress relative to that of wild-type plants, which indicated a negative role of OsATL38 in response to cold stress. Yeast two-hybrid, in vitro pull-down, and co-immunoprecipitation assays revealed that OsATL38 physically interacted with OsGF14d, a rice 14-3-3 protein. An in vivo target ubiquitination assay indicated that OsGF14d was mono-ubiquitinated by OsATL38. osgf14d knockout mutant plants were more sensitive to cold stress than wild-type rice plants, indicating that OsGF14d is a positive factor in the response to cold stress. These results provide evidence that the RING E3 Ub ligase OsATL38 negatively regulates the cold stress response in rice via mono-ubiquitination of OsGF14d 14-3-3 protein.

Original languageEnglish
Pages (from-to)307-323
Number of pages17
JournalJournal of experimental botany
Issue number1
Publication statusPublished - 2022 Jan 5

Bibliographical note

Funding Information:
This work was supported by grants from the Next-Generation BioGreen 21 Program for Agriculture and Technology Development [Project No. PJ01334901 (SSAC)] funded by the Rural Development Administration and Basic Science Research Program, Project No. 2018R1A6A1A03025607 through the National Research Foundation (NRF) funded by the Ministry of Education, Republic of Korea to WTK. SGY was the recipient of a Hyundai Motor Chung Mong-Koo Foundation Scholarship.

Publisher Copyright:
© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved.

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science


Dive into the research topics of 'OsATL38 mediates mono-ubiquitination of the 14-3-3 protein OsGF14d and negatively regulates the cold stress response in rice'. Together they form a unique fingerprint.

Cite this