OsEIN2 is a positive component in ethylene signaling in rice

Sung Hoon Jun, Min Jung Han, Shinyoung Lee, Young Sam Seo, Woo Taek Kim, Gynheung An

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

EIN2 is a central signal transducer in the ethylene-signaling pathway, and a unique membrane-anchored protein. By screening a cDNA library, we have isolated a cDNA clone (OsEIN2) that encodes the rice EIN2 homolog. The full-length ORF clone was obtained by reverse transcriptase-polymerase chain reaction. OsEIN2 shares significant amino acid sequence similarity with Arabidopsis EIN2 (57% similarity and 42% identity). Both the numbers and positions of introns and exons in the OsEIN2 and AtEIN2 coding regions are also conserved. To address whether this structural similarity is indicative of functional conservation of the corresponding proteins, we also generated transgenic lines expressing the antisense construct of OsEIN2. Those plants were stunted and shoot elongation was severely inhibited. Their phenotypes were similar to that found with wild-type rice seedlings that were treated with AgNO3, an ethylene signal inhibitor. In the OsEIN2 antisense plants, the expression levels of two ethylene-responsive genes, SC129 and SC255, were decreased compared with the wild types. These results suggest that OsEIN2 is a positive component of the ethylene-signaling pathway in rice, just as AtEIN2 is in Arabidopsis. Our antisense transgenic plants produced approximately 3.5 times more ethylene than the wild-type plants. Expression analysis of rice ACS and ACO genes showed that the transcript levels of OsACS1 and OsACO1 were elevated in the transgenic plants.

Original languageEnglish
Pages (from-to)281-289
Number of pages9
JournalPlant and Cell Physiology
Volume45
Issue number3
DOIs
Publication statusPublished - 2004 Mar 1

Fingerprint

ethylene
rice
Genetically Modified Plants
Arabidopsis
transgenic plants
Clone Cells
clones
Reverse Transcriptase Polymerase Chain Reaction
Transducers
Gene Library
Seedlings
cDNA libraries
Introns
Open Reading Frames
Genes
exons
open reading frames
introns
Amino Acid Sequence
Exons

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Jun, Sung Hoon ; Han, Min Jung ; Lee, Shinyoung ; Seo, Young Sam ; Kim, Woo Taek ; An, Gynheung. / OsEIN2 is a positive component in ethylene signaling in rice. In: Plant and Cell Physiology. 2004 ; Vol. 45, No. 3. pp. 281-289.
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OsEIN2 is a positive component in ethylene signaling in rice. / Jun, Sung Hoon; Han, Min Jung; Lee, Shinyoung; Seo, Young Sam; Kim, Woo Taek; An, Gynheung.

In: Plant and Cell Physiology, Vol. 45, No. 3, 01.03.2004, p. 281-289.

Research output: Contribution to journalArticle

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