Osteogenic activity of chitosan-based hybrid scaffold prepared by polyelectrolyte complex formation with alginate

Seung Hwan Lee, Ho Yun Chung, Hong In Shin, Dong Joon Park, Jin Hyun Choi

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8 Citations (Scopus)


A chitosan (CS)/alginate (AG) hybrid scaffold was prepared through solid state polyelectrolyte complex (PEC) formation. The PEC formation was confirmed by Fourier-transformed infrared spectroscopy. Positive von Kossa staining of calcium depositions appeared in a large area of the CS/AG PEC scaffold cultured with osteoblastlike KUSA-A1 cells. The alkaline phosphatase activity of the cells cultured in the CS/AG scaffold was higher than that of the cells in the CS/hydroxyapatite (HA) scaffold. The osteogenic activity of the CS/AG PEC scaffold was considered comparable to that of the CS/HA scaffold. These findings suggest that modification of CS-based scaffold by PEC formation have a significant effect on the cell behavior towards osteogenic differentiation and bone tissue regeneration.

Original languageEnglish
Pages (from-to)106-112
Number of pages7
JournalTissue Engineering and Regenerative Medicine
Issue number2
Publication statusPublished - 2014 Jan 1


All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomedical Engineering

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