Osteogenic potency of nacre on human mesenchymal stem cells

David W. Green, Hyuk Jae Kwon, Hansung Jung

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Nacre seashell is a natural osteoinductive biomaterial with strong effects on osteoprogenitors, osteoblasts, and osteoclasts during bone tissue formation and morphogenesis. Although nacre has shown, in one study, to induce bridging of new bone across large non-union bone defects in 8 individual human patients, there have been no succeeding human surgical studies to confirm this outstanding potency. But the molecular mechanisms associated with nacre osteoinduction and the influence on bone marrow-derived mesenchymal stem cells (BMSC’s), skeletal stem cells or bone marrow stromal cells remain elusive. In this study we highlight the phenotypic and biochemical effects of Pinctada maxima nacre chips and the global nacre soluble protein matrix (SPM) on primary human bone marrowderived stromal cells (hBMSCs) in vitro. In static co-culture with nacre chips, the hBMSCs secreted Alkaline phosphatase (ALP) at levels that exceeded bone morphogenetic protein (rhBMP-2) treatment. Concentrated preparation of SPM applied to Stro-1 selected hBMSC’s led to rapid ALP secretions, at concentrations exceeding the untreated controls even in osteogenic conditions. Within 21 days the same population of Stro-1 selected hBMSCs proliferated and secreted collagens I-IV, indicating the premature onset of an osteoblast phenotype. The same SPM was found to promote unselected hBMSC differentiation with osteocalcin detected at 7 days, and proliferation increased at 7 days in a dose-dependent manner. In conclusion, nacre particles and nacre SPM induced the early stages of human bone cell differentiation, indicating that they may be promising soluble factors with osteoinductive capacity in primary human bone cell progenitors such as, hBMSC’s.

Original languageEnglish
Pages (from-to)267-272
Number of pages6
JournalMolecules and Cells
Volume38
Issue number3
DOIs
Publication statusPublished - 2015 Feb 5

Fingerprint

Nacre
Mesenchymal Stromal Cells
Bone and Bones
Stromal Cells
Osteoblasts
Animal Shells
Alkaline Phosphatase
Cell Differentiation
Pinctada
Proteins
Stem Cells
Bone Morphogenetic Protein 2
Osteocalcin
Biocompatible Materials
Osteoclasts
Coculture Techniques
Morphogenesis
Osteogenesis

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Green, David W. ; Kwon, Hyuk Jae ; Jung, Hansung. / Osteogenic potency of nacre on human mesenchymal stem cells. In: Molecules and Cells. 2015 ; Vol. 38, No. 3. pp. 267-272.
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Osteogenic potency of nacre on human mesenchymal stem cells. / Green, David W.; Kwon, Hyuk Jae; Jung, Hansung.

In: Molecules and Cells, Vol. 38, No. 3, 05.02.2015, p. 267-272.

Research output: Contribution to journalArticle

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