Osteogenic priming of mesenchymal stem cells by chondrocyte-conditioned factors and mineralized matrix

Hyunuk Ro, Jungha Park, Kisuk Yang, Jiyong Kim, Hyun Gu Yim, Giyoung Jung, Hyukjin Lee, Seung Woo Cho, Nathaniel S. Hwang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Transient cartilage and a mineralizing microenvironment play pivotal roles in mesenchymal cell ossification during bone formation. In order to recreate these microenvironmental cues, C3H10T1/2 murine mesenchymal stem cells (MSCs) were exposed to chondrocyte-conditioned medium (CM) and seeded onto three-dimensional mineralized scaffolds for bone regeneration. Expansion of C3H10T1/2 cells with CM resulted in enhanced expression levels of chondrogenic markers such as aggrecan, type II collagen, type X collagen, and Sox9, rather than of osteogenic genes. Interestingly, CM expansion led to reduced expression levels of osteogenic genes such as alkaline phosphatase (ALP), type I collagen, osteocalcin, and Runx2. However, CM-expanded C3H10T1/2 cells showed enhanced osteogenic differentiation as indicated by increased ALP and Alizarin Red S staining upon osteogenic factor exposure. In vivo, CM-expanded C3H10T1/2 mesenchymal cells were seeded onto mineralized scaffolds (fabricated with polydopamine and coated with simulated body fluids) and implanted into critical-sized calvarial-defect mouse models. After 8 weeks of implantation, mouse skulls were collected, and bone tissue regeneration was evaluated by micro-computed tumography and Masson’s trichrome staining. In accordance with the in vitro analysis, CM-expanded C3H10T1/2 cells gave enhanced bone mineral deposition. Thus, chondrocyte-conditioned factors and a mineralized microenvironment stimulate the bone formation of MSCs.

Original languageEnglish
Pages (from-to)115-126
Number of pages12
JournalCell and Tissue Research
Volume362
Issue number1
DOIs
Publication statusPublished - 2015 Oct 22

Fingerprint

Conditioned Culture Medium
Chondrocytes
Mesenchymal Stromal Cells
Osteogenesis
Bone Regeneration
Alkaline Phosphatase
Collagen Type X
Staining and Labeling
Aggrecans
Bone and Bones
Osteocalcin
Body Fluids
Collagen Type I
Skull
Genes
Cartilage
Cues
Minerals
Collagen

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Ro, Hyunuk ; Park, Jungha ; Yang, Kisuk ; Kim, Jiyong ; Yim, Hyun Gu ; Jung, Giyoung ; Lee, Hyukjin ; Cho, Seung Woo ; Hwang, Nathaniel S. / Osteogenic priming of mesenchymal stem cells by chondrocyte-conditioned factors and mineralized matrix. In: Cell and Tissue Research. 2015 ; Vol. 362, No. 1. pp. 115-126.
@article{a65a38accf8f4b12935cb48ac77ca48c,
title = "Osteogenic priming of mesenchymal stem cells by chondrocyte-conditioned factors and mineralized matrix",
abstract = "Transient cartilage and a mineralizing microenvironment play pivotal roles in mesenchymal cell ossification during bone formation. In order to recreate these microenvironmental cues, C3H10T1/2 murine mesenchymal stem cells (MSCs) were exposed to chondrocyte-conditioned medium (CM) and seeded onto three-dimensional mineralized scaffolds for bone regeneration. Expansion of C3H10T1/2 cells with CM resulted in enhanced expression levels of chondrogenic markers such as aggrecan, type II collagen, type X collagen, and Sox9, rather than of osteogenic genes. Interestingly, CM expansion led to reduced expression levels of osteogenic genes such as alkaline phosphatase (ALP), type I collagen, osteocalcin, and Runx2. However, CM-expanded C3H10T1/2 cells showed enhanced osteogenic differentiation as indicated by increased ALP and Alizarin Red S staining upon osteogenic factor exposure. In vivo, CM-expanded C3H10T1/2 mesenchymal cells were seeded onto mineralized scaffolds (fabricated with polydopamine and coated with simulated body fluids) and implanted into critical-sized calvarial-defect mouse models. After 8 weeks of implantation, mouse skulls were collected, and bone tissue regeneration was evaluated by micro-computed tumography and Masson’s trichrome staining. In accordance with the in vitro analysis, CM-expanded C3H10T1/2 cells gave enhanced bone mineral deposition. Thus, chondrocyte-conditioned factors and a mineralized microenvironment stimulate the bone formation of MSCs.",
author = "Hyunuk Ro and Jungha Park and Kisuk Yang and Jiyong Kim and Yim, {Hyun Gu} and Giyoung Jung and Hyukjin Lee and Cho, {Seung Woo} and Hwang, {Nathaniel S.}",
year = "2015",
month = "10",
day = "22",
doi = "10.1007/s00441-015-2195-7",
language = "English",
volume = "362",
pages = "115--126",
journal = "Cell and Tissue Research",
issn = "0302-766X",
publisher = "Springer Verlag",
number = "1",

}

Osteogenic priming of mesenchymal stem cells by chondrocyte-conditioned factors and mineralized matrix. / Ro, Hyunuk; Park, Jungha; Yang, Kisuk; Kim, Jiyong; Yim, Hyun Gu; Jung, Giyoung; Lee, Hyukjin; Cho, Seung Woo; Hwang, Nathaniel S.

In: Cell and Tissue Research, Vol. 362, No. 1, 22.10.2015, p. 115-126.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Osteogenic priming of mesenchymal stem cells by chondrocyte-conditioned factors and mineralized matrix

AU - Ro, Hyunuk

AU - Park, Jungha

AU - Yang, Kisuk

AU - Kim, Jiyong

AU - Yim, Hyun Gu

AU - Jung, Giyoung

AU - Lee, Hyukjin

AU - Cho, Seung Woo

AU - Hwang, Nathaniel S.

PY - 2015/10/22

Y1 - 2015/10/22

N2 - Transient cartilage and a mineralizing microenvironment play pivotal roles in mesenchymal cell ossification during bone formation. In order to recreate these microenvironmental cues, C3H10T1/2 murine mesenchymal stem cells (MSCs) were exposed to chondrocyte-conditioned medium (CM) and seeded onto three-dimensional mineralized scaffolds for bone regeneration. Expansion of C3H10T1/2 cells with CM resulted in enhanced expression levels of chondrogenic markers such as aggrecan, type II collagen, type X collagen, and Sox9, rather than of osteogenic genes. Interestingly, CM expansion led to reduced expression levels of osteogenic genes such as alkaline phosphatase (ALP), type I collagen, osteocalcin, and Runx2. However, CM-expanded C3H10T1/2 cells showed enhanced osteogenic differentiation as indicated by increased ALP and Alizarin Red S staining upon osteogenic factor exposure. In vivo, CM-expanded C3H10T1/2 mesenchymal cells were seeded onto mineralized scaffolds (fabricated with polydopamine and coated with simulated body fluids) and implanted into critical-sized calvarial-defect mouse models. After 8 weeks of implantation, mouse skulls were collected, and bone tissue regeneration was evaluated by micro-computed tumography and Masson’s trichrome staining. In accordance with the in vitro analysis, CM-expanded C3H10T1/2 cells gave enhanced bone mineral deposition. Thus, chondrocyte-conditioned factors and a mineralized microenvironment stimulate the bone formation of MSCs.

AB - Transient cartilage and a mineralizing microenvironment play pivotal roles in mesenchymal cell ossification during bone formation. In order to recreate these microenvironmental cues, C3H10T1/2 murine mesenchymal stem cells (MSCs) were exposed to chondrocyte-conditioned medium (CM) and seeded onto three-dimensional mineralized scaffolds for bone regeneration. Expansion of C3H10T1/2 cells with CM resulted in enhanced expression levels of chondrogenic markers such as aggrecan, type II collagen, type X collagen, and Sox9, rather than of osteogenic genes. Interestingly, CM expansion led to reduced expression levels of osteogenic genes such as alkaline phosphatase (ALP), type I collagen, osteocalcin, and Runx2. However, CM-expanded C3H10T1/2 cells showed enhanced osteogenic differentiation as indicated by increased ALP and Alizarin Red S staining upon osteogenic factor exposure. In vivo, CM-expanded C3H10T1/2 mesenchymal cells were seeded onto mineralized scaffolds (fabricated with polydopamine and coated with simulated body fluids) and implanted into critical-sized calvarial-defect mouse models. After 8 weeks of implantation, mouse skulls were collected, and bone tissue regeneration was evaluated by micro-computed tumography and Masson’s trichrome staining. In accordance with the in vitro analysis, CM-expanded C3H10T1/2 cells gave enhanced bone mineral deposition. Thus, chondrocyte-conditioned factors and a mineralized microenvironment stimulate the bone formation of MSCs.

UR - http://www.scopus.com/inward/record.url?scp=84941877298&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941877298&partnerID=8YFLogxK

U2 - 10.1007/s00441-015-2195-7

DO - 10.1007/s00441-015-2195-7

M3 - Article

C2 - 25956591

AN - SCOPUS:84941877298

VL - 362

SP - 115

EP - 126

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

IS - 1

ER -