Outbreak of KPC-2-producing Enterobacteriaceae caused by clonal dissemination of Klebsiella pneumoniae ST307 carrying an IncX3-type plasmid harboring a truncated Tn4401a

Jung Ok Kim, Sae Am Song, Eun Jeong Yoon, Jeong Hwan Shin, Hyukmin Lee, Seok Hoon Jeong, Kyungwon Lee

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Over a 5-month period between the end of June and the beginning of November in 2015, a KPC-producing Enterobacteriaceae outbreak occurred in a general hospital in Busan, South Korea, being associated with a total of 50 clinical isolates from 47 patients. Multilocus sequence typing and pulsed-field gel electrophoresis were carried out for strain typing and whole-genome sequencing was performed to characterize the plasmids. A clonal spread of K. pneumoniae sequence type 307 (ST307) carrying a self-transferable IncX3-type plasmid harboring blaKPC-2 was responsible for the outbreak. Sporadic emergence of K. pneumoniae ST697 carrying an IncFII-type plasmid and a ST11 isolate harboring a small plasmid devoid of any known origin of replication were observed to be associated with blaKPC-3, but no further dissemination of these strains was identified. The results indicated a healthcare-associated infection associated with a blaKPC-harboring plasmid dissemination and a clonal spread of KPC-producing Enterobacteriaceae.

Original languageEnglish
Pages (from-to)343-348
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume87
Issue number4
DOIs
Publication statusPublished - 2017 Apr 1

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Klebsiella pneumoniae
Enterobacteriaceae
Disease Outbreaks
Plasmids
Multilocus Sequence Typing
Republic of Korea
Replication Origin
Pulsed Field Gel Electrophoresis
Cross Infection
General Hospitals
Genome

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

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title = "Outbreak of KPC-2-producing Enterobacteriaceae caused by clonal dissemination of Klebsiella pneumoniae ST307 carrying an IncX3-type plasmid harboring a truncated Tn4401a",
abstract = "Over a 5-month period between the end of June and the beginning of November in 2015, a KPC-producing Enterobacteriaceae outbreak occurred in a general hospital in Busan, South Korea, being associated with a total of 50 clinical isolates from 47 patients. Multilocus sequence typing and pulsed-field gel electrophoresis were carried out for strain typing and whole-genome sequencing was performed to characterize the plasmids. A clonal spread of K. pneumoniae sequence type 307 (ST307) carrying a self-transferable IncX3-type plasmid harboring blaKPC-2 was responsible for the outbreak. Sporadic emergence of K. pneumoniae ST697 carrying an IncFII-type plasmid and a ST11 isolate harboring a small plasmid devoid of any known origin of replication were observed to be associated with blaKPC-3, but no further dissemination of these strains was identified. The results indicated a healthcare-associated infection associated with a blaKPC-harboring plasmid dissemination and a clonal spread of KPC-producing Enterobacteriaceae.",
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Outbreak of KPC-2-producing Enterobacteriaceae caused by clonal dissemination of Klebsiella pneumoniae ST307 carrying an IncX3-type plasmid harboring a truncated Tn4401a. / Kim, Jung Ok; Song, Sae Am; Yoon, Eun Jeong; Shin, Jeong Hwan; Lee, Hyukmin; Jeong, Seok Hoon; Lee, Kyungwon.

In: Diagnostic Microbiology and Infectious Disease, Vol. 87, No. 4, 01.04.2017, p. 343-348.

Research output: Contribution to journalArticle

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