Overexpression and spectroscopic characterization of a recombinant human tumor suppressor p16INK4

Weontae Lee; Ji Uk Jang; Dong Myeong Kim; Hosun Son; Beom Seok Yang

Overexpression and spectroscopic characterization of a recombinant human tumor suppressor p16^INK4

Weontae Lee, Ji Uk Jang, Dong Myeong Kim, Hosun Son, Beom Seok Yang

Research output: Contribution to journal › Article

Abstract

p16^INK4, which is a 16-kDa polypeptide protein, inhibits the catalytic activity of the CDK4-cyclinD complex to suppress tumor growth. Both unlabeled and isotopelabeled human tumor suppressor p16^INK4 protein were overexpressed and purified to characterize biochemical and structural properties. The purified p16 binds to monomeric GST-CDK4 and exists in a monomer conformation for several weeks at 4°C. The circular dichroism (CD) data indicates that p16 contains high percentage of α-helix and that the helix percentage maximized at pH value of 7.0. One- and two-dimensional nuclear magnetic resonance (NMR) data suggest that purified p16 from our construct has a unique folded conformation under our experimental conditions and exhibits quite stable conformational characteristics.

Original language	English
Pages (from-to)	48-52
Number of pages	5
Journal	Journal of biochemistry and molecular biology
Volume	31
Issue number	1
Publication status	Published - 1998 Jan 31

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology

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Lee, W., Jang, J. U., Kim, D. M., Son, H., & Yang, B. S. (1998). Overexpression and spectroscopic characterization of a recombinant human tumor suppressor p16^INK4. Journal of biochemistry and molecular biology, 31(1), 48-52.

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AU - Yang, Beom Seok

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