Overexpression of a brassica rapa NGATHA gene in arabidopsis thaliana negatively affects cell proliferation during lateral organ and root growth

So Hyun Kwon, Byung Ha Lee, Eun Yu Kim, Young Sam Seo, Sangman Lee, Woo Taek Kim, Jong Tae Song, Jeong Hoe Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In an effort to elucidate biological functions of transcription factors of Brassica rapa L. (ssp. pekinensis), an NGATHA homolog, BrNGA1, that belongs to the B3-type transcription factor superfamily was identified and expressed in Arabidopsis thaliana under the control of the cauliflower mosaic virus (CaMV) 35S promoter. Arabidopsis plants overexpressing BrNGA1, named BrNGA1ox, displayed markedly reduced organ growth compared with the wild type: lateral organs, such as leaves, flowers and cotyledons, were small and distinctively narrow, and their root growth was also severely retarded. Reduced sizes of BrNGA1ox organs were mainly due to reduction in cell numbers. Kinematic analysis of leaf growth revealed that both the rate and duration of cell proliferation declined during organogenesis, which was consistent with the reduced expression of cyclin genes. Reduction in organ growth was strongly correlated with the small size of meristematic cell pools in the shoot and root meristems. Taken together, these data indicate that BrNGA1 acts as a negative regulator of cell proliferation and may do so, in part, by regulating the size of the meristematic cell pool.

Original languageEnglish
Pages (from-to)2163-2173
Number of pages11
JournalPlant and Cell Physiology
Volume50
Issue number12
DOIs
Publication statusPublished - 2009 Dec 1

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Brassica rapa
Arabidopsis
root growth
cell proliferation
Arabidopsis thaliana
Cell Proliferation
transcription factors
Growth
Cell Size
Genes
Transcription Factors
genes
root meristems
Cauliflower mosaic virus
shoot meristems
cyclins
cells
Caulimovirus
organogenesis
kinematics

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Kwon, So Hyun ; Lee, Byung Ha ; Kim, Eun Yu ; Seo, Young Sam ; Lee, Sangman ; Kim, Woo Taek ; Song, Jong Tae ; Kim, Jeong Hoe. / Overexpression of a brassica rapa NGATHA gene in arabidopsis thaliana negatively affects cell proliferation during lateral organ and root growth. In: Plant and Cell Physiology. 2009 ; Vol. 50, No. 12. pp. 2163-2173.
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Overexpression of a brassica rapa NGATHA gene in arabidopsis thaliana negatively affects cell proliferation during lateral organ and root growth. / Kwon, So Hyun; Lee, Byung Ha; Kim, Eun Yu; Seo, Young Sam; Lee, Sangman; Kim, Woo Taek; Song, Jong Tae; Kim, Jeong Hoe.

In: Plant and Cell Physiology, Vol. 50, No. 12, 01.12.2009, p. 2163-2173.

Research output: Contribution to journalArticle

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