Overexpression of a chromatin architecture-controlling AT-hook protein extends leaf longevity and increases the post-harvest storage life of plants

Pyung Ok Lim, Yumi Kim, Emily Breeze, Ja Choon Koo, Hye Ryun Woo, Jong Sang Ryu, Donha Park, Jim Beynon, Alex Tabrett, Vicky Buchanan-Wollaston, Hong Gil Nam

Research output: Contribution to journalArticle

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Abstract

Leaf senescence is the final stage of leaf development and is finely regulated via a complex genetic regulatory network incorporating both developmental and environmental factors. In an effort to identify negative regulators of leaf senescence, we screened activation-tagged Arabidopsis lines for mutants that exhibit a delayed leaf senescence phenotype. One of the mutants (ore7-1D) showed a highly significant delay of leaf senescence in the heterozygous state, leading to at least a twofold increase in leaf longevity. The activated gene (ORE7/ESC) encoded a protein with an AT-hook DNA-binding motif; such proteins are known to co-regulate transcription of genes through modification of chromatin architecture. We showed that ORE7/ESC, in addition to binding to a plant AT-rich DNA fragment, could also modify the chromatin architecture, as illustrated by an altered distribution of a histone-GFP fusion protein in the nucleus of the mutant. Globally altered gene expression, shown by microarray analysis, also indicated that activation of ORE7/ESC results in a younger condition in the mutant leaves. We propose that ectopically expressed ORE7/ESC is negatively regulating leaf senescence and suggest that the resulting chromatin alteration may have a role in controlling leaf longevity. Interestingly, activation of ORE7/ESC also led to a highly extended post-harvest storage life.

Original languageEnglish
Pages (from-to)1140-1153
Number of pages14
JournalPlant Journal
Volume52
Issue number6
DOIs
Publication statusPublished - 2007 Dec 1

Fingerprint

AT-Hook Motifs
leaf protein
Chromatin
chromatin
shelf life
leaves
Proteins
mutants
Nucleotide Motifs
Microarray Analysis
Arabidopsis
Histones
Genes
DNA-binding domains
proteins
leaf development
Phenotype
Gene Expression
histones
DNA

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Lim, Pyung Ok ; Kim, Yumi ; Breeze, Emily ; Koo, Ja Choon ; Woo, Hye Ryun ; Ryu, Jong Sang ; Park, Donha ; Beynon, Jim ; Tabrett, Alex ; Buchanan-Wollaston, Vicky ; Nam, Hong Gil. / Overexpression of a chromatin architecture-controlling AT-hook protein extends leaf longevity and increases the post-harvest storage life of plants. In: Plant Journal. 2007 ; Vol. 52, No. 6. pp. 1140-1153.
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abstract = "Leaf senescence is the final stage of leaf development and is finely regulated via a complex genetic regulatory network incorporating both developmental and environmental factors. In an effort to identify negative regulators of leaf senescence, we screened activation-tagged Arabidopsis lines for mutants that exhibit a delayed leaf senescence phenotype. One of the mutants (ore7-1D) showed a highly significant delay of leaf senescence in the heterozygous state, leading to at least a twofold increase in leaf longevity. The activated gene (ORE7/ESC) encoded a protein with an AT-hook DNA-binding motif; such proteins are known to co-regulate transcription of genes through modification of chromatin architecture. We showed that ORE7/ESC, in addition to binding to a plant AT-rich DNA fragment, could also modify the chromatin architecture, as illustrated by an altered distribution of a histone-GFP fusion protein in the nucleus of the mutant. Globally altered gene expression, shown by microarray analysis, also indicated that activation of ORE7/ESC results in a younger condition in the mutant leaves. We propose that ectopically expressed ORE7/ESC is negatively regulating leaf senescence and suggest that the resulting chromatin alteration may have a role in controlling leaf longevity. Interestingly, activation of ORE7/ESC also led to a highly extended post-harvest storage life.",
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Lim, PO, Kim, Y, Breeze, E, Koo, JC, Woo, HR, Ryu, JS, Park, D, Beynon, J, Tabrett, A, Buchanan-Wollaston, V & Nam, HG 2007, 'Overexpression of a chromatin architecture-controlling AT-hook protein extends leaf longevity and increases the post-harvest storage life of plants', Plant Journal, vol. 52, no. 6, pp. 1140-1153. https://doi.org/10.1111/j.1365-313X.2007.03317.x

Overexpression of a chromatin architecture-controlling AT-hook protein extends leaf longevity and increases the post-harvest storage life of plants. / Lim, Pyung Ok; Kim, Yumi; Breeze, Emily; Koo, Ja Choon; Woo, Hye Ryun; Ryu, Jong Sang; Park, Donha; Beynon, Jim; Tabrett, Alex; Buchanan-Wollaston, Vicky; Nam, Hong Gil.

In: Plant Journal, Vol. 52, No. 6, 01.12.2007, p. 1140-1153.

Research output: Contribution to journalArticle

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T1 - Overexpression of a chromatin architecture-controlling AT-hook protein extends leaf longevity and increases the post-harvest storage life of plants

AU - Lim, Pyung Ok

AU - Kim, Yumi

AU - Breeze, Emily

AU - Koo, Ja Choon

AU - Woo, Hye Ryun

AU - Ryu, Jong Sang

AU - Park, Donha

AU - Beynon, Jim

AU - Tabrett, Alex

AU - Buchanan-Wollaston, Vicky

AU - Nam, Hong Gil

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N2 - Leaf senescence is the final stage of leaf development and is finely regulated via a complex genetic regulatory network incorporating both developmental and environmental factors. In an effort to identify negative regulators of leaf senescence, we screened activation-tagged Arabidopsis lines for mutants that exhibit a delayed leaf senescence phenotype. One of the mutants (ore7-1D) showed a highly significant delay of leaf senescence in the heterozygous state, leading to at least a twofold increase in leaf longevity. The activated gene (ORE7/ESC) encoded a protein with an AT-hook DNA-binding motif; such proteins are known to co-regulate transcription of genes through modification of chromatin architecture. We showed that ORE7/ESC, in addition to binding to a plant AT-rich DNA fragment, could also modify the chromatin architecture, as illustrated by an altered distribution of a histone-GFP fusion protein in the nucleus of the mutant. Globally altered gene expression, shown by microarray analysis, also indicated that activation of ORE7/ESC results in a younger condition in the mutant leaves. We propose that ectopically expressed ORE7/ESC is negatively regulating leaf senescence and suggest that the resulting chromatin alteration may have a role in controlling leaf longevity. Interestingly, activation of ORE7/ESC also led to a highly extended post-harvest storage life.

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