Overexpression of bcl-2 attenuates mpp + , but not 6-odha, induced cell death in a dopaminergic neuronal cell line

Young Jun Oh, Shou C. Wong, Mark Moffat, Karen L. O’Malley

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

In order to investigate the role of Bcl-2 in dopaminergic cells, we established a dopaminergic neuronal cell line (MN9D) stably expressing human Bcl-2 (MN9D/Bcl-2) or neomycin (MN9D/Neo). Overexpression of Bcl-2 in MN9D cells attenuated cell death due to treatment of mitochondrial electron transport inhibitors including N-methyl-4-phenylpyridinium, whereas it did not prevent cell death induced by reagents generating reactive oxygen species including 6-hydroxy-dopamine. Moreover, the rate of glucose uptake in MN9D/Bcl-2 was significantly lower than that in MN9D/Neo after MPP + treatment. Thus, Bcl-2 may counter aberrations in mitochondrial electron transfer processes by altering energy metabolism within the MN9D cells.

Original languageEnglish
Pages (from-to)157-167
Number of pages11
JournalNeurobiology of Disease
Volume2
Issue number3
DOIs
Publication statusPublished - 1995 Jan 1

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Cell Death
Neomycin
Cell Line
1-Methyl-4-phenylpyridinium
Electron Transport
Energy Metabolism
Reactive Oxygen Species
Dopamine
Electrons
Glucose

All Science Journal Classification (ASJC) codes

  • Neurology

Cite this

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abstract = "In order to investigate the role of Bcl-2 in dopaminergic cells, we established a dopaminergic neuronal cell line (MN9D) stably expressing human Bcl-2 (MN9D/Bcl-2) or neomycin (MN9D/Neo). Overexpression of Bcl-2 in MN9D cells attenuated cell death due to treatment of mitochondrial electron transport inhibitors including N-methyl-4-phenylpyridinium, whereas it did not prevent cell death induced by reagents generating reactive oxygen species including 6-hydroxy-dopamine. Moreover, the rate of glucose uptake in MN9D/Bcl-2 was significantly lower than that in MN9D/Neo after MPP + treatment. Thus, Bcl-2 may counter aberrations in mitochondrial electron transfer processes by altering energy metabolism within the MN9D cells.",
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Overexpression of bcl-2 attenuates mpp + , but not 6-odha, induced cell death in a dopaminergic neuronal cell line . / Oh, Young Jun; Wong, Shou C.; Moffat, Mark; O’Malley, Karen L.

In: Neurobiology of Disease, Vol. 2, No. 3, 01.01.1995, p. 157-167.

Research output: Contribution to journalArticle

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