Overexpression of bcl-2, bcl-x(L) or hsp70 in murine cortical astrocytes reduces injury of co-cultured neurons

Lijun Xu; Jong Eun Lee; Rona G. Giffard

doi:10.1016/S0304-3940(99)00882-4

Overexpression of bcl-2, bcl-x(L) or hsp70 in murine cortical astrocytes reduces injury of co-cultured neurons

Lijun Xu, Jong Eun Lee, Rona G. Giffard

Department of Anatomy

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

Astrocytes perform many functions that protect neurons during stress, including transmitter uptake, metabolic support, and protection from oxidative stress. We asked whether astrocytes overexpressing either the anti-apoptotic genes bcl-2, or bcl-x(L), or the inducible heat shock protein hsp70, could better protect neurons grown with them in co-culture than normal astrocytes or astrocytes expressing β-galactosidase. Retroviral vectors were used to express these genes in primary astrocyte cultures. After antibiotic selection to eliminate untransformed astrocytes, neurons were plated on top of the astrocytes. Overexpression of any of the three genes in astrocytes reduced neuronal injury induced by combined oxygen-glucose deprivation, or glucose deprivation. Hsp70 overexpression reduced glutamate toxicity. As none of the genes studied is thought to be secreted, the likeliest explanation for the protection observed is improved astrocyte function. Copyright (C) 1999.

Original language	English
Pages (from-to)	193-197
Number of pages	5
Journal	Neuroscience Letters
Volume	277
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(99)00882-4
Publication status	Published - 1999 Dec 31

Bibliographical note

Funding Information:
The authors thank Nina Radford for providing the hsp70 transgenic mice and Dr. David Vaux for the bcl-2 and lac-z retrovirus. The work was supported in part by NIH grant GM49831 and a Frontiers in Anesthesia Research Award from the International Anesthesia Research Society to R.G.G.

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Neuroscience(all)

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title = "Overexpression of bcl-2, bcl-x(L) or hsp70 in murine cortical astrocytes reduces injury of co-cultured neurons",

abstract = "Astrocytes perform many functions that protect neurons during stress, including transmitter uptake, metabolic support, and protection from oxidative stress. We asked whether astrocytes overexpressing either the anti-apoptotic genes bcl-2, or bcl-x(L), or the inducible heat shock protein hsp70, could better protect neurons grown with them in co-culture than normal astrocytes or astrocytes expressing β-galactosidase. Retroviral vectors were used to express these genes in primary astrocyte cultures. After antibiotic selection to eliminate untransformed astrocytes, neurons were plated on top of the astrocytes. Overexpression of any of the three genes in astrocytes reduced neuronal injury induced by combined oxygen-glucose deprivation, or glucose deprivation. Hsp70 overexpression reduced glutamate toxicity. As none of the genes studied is thought to be secreted, the likeliest explanation for the protection observed is improved astrocyte function. Copyright (C) 1999.",

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N1 - Funding Information: The authors thank Nina Radford for providing the hsp70 transgenic mice and Dr. David Vaux for the bcl-2 and lac-z retrovirus. The work was supported in part by NIH grant GM49831 and a Frontiers in Anesthesia Research Award from the International Anesthesia Research Society to R.G.G.

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AB - Astrocytes perform many functions that protect neurons during stress, including transmitter uptake, metabolic support, and protection from oxidative stress. We asked whether astrocytes overexpressing either the anti-apoptotic genes bcl-2, or bcl-x(L), or the inducible heat shock protein hsp70, could better protect neurons grown with them in co-culture than normal astrocytes or astrocytes expressing β-galactosidase. Retroviral vectors were used to express these genes in primary astrocyte cultures. After antibiotic selection to eliminate untransformed astrocytes, neurons were plated on top of the astrocytes. Overexpression of any of the three genes in astrocytes reduced neuronal injury induced by combined oxygen-glucose deprivation, or glucose deprivation. Hsp70 overexpression reduced glutamate toxicity. As none of the genes studied is thought to be secreted, the likeliest explanation for the protection observed is improved astrocyte function. Copyright (C) 1999.

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Overexpression of bcl-2, bcl-x(L) or hsp70 in murine cortical astrocytes reduces injury of co-cultured neurons

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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