A dopaminergic neuronal cell line was established which stably expressed human Bcl-2 (MN9D/Bcl-2) or a control vector (MN9D/ Neo). Overexpression of Bcl-2 cells led to robust neurite formation without cessation of cell division. Most markers of neuronal and/or dopaminergic maturation were unchanged although the synaptosomal associated protein, SNAP-25, was increased three-fold in MN9D/ Bcl-2 cell lines. These data support a role for Bcl-2 in developmental processes at least during a certain stage(s) of neurogenesis.
Bibliographical noteFunding Information:
We thank Dr. Richard D. Todd for thoughtful discussions and critical reading of parts of this manuscript, and Dr. C. O'Hara for technical assistance. We also thank Drs. Heller, Hoffman and Won for providing us with the MN9D cells and for the ongoing collaboration between ourselves. A portion of this work is published in abstract form. Supported by NIA 44626, NS54982, MH45530.
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