Overexpression of human arginine decarboxylase rescues human mesenchymal stem cells against H2O2 toxicity through cell survival protein activation

Su Kyoung Seo, Wonsuk Yang, Yu Mi Park, Won Taek Lee, Kyung Ah Park, Jong Eun Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In this study, we explored the potentiality of human arginine decarboxylase (ADC) to enhance the survival of mesenchymal stem cells (MSCs) against unfavorable milieu of host tissues as the low survival of MSCs is the issue in cell transplantation therapy. To address this, human MSCs overexpressing human ADC were treated with H2O2 and the resultant intracellular events were examined. First, we examined whether human ADC is overexpressed in human MSCs. Then, we investigated cell survival or death related events. We found that the overexpression of human ADC increases formazan production and reduces caspase 3 activation and the numbers of FITC, hoechst, or propidium iodide positive cells in human MSCs exposed to H2O2. To elucidate the factors underlying these phenomena, AKT, CREB, and BDNF were examined. We found that the overexpression of human ADC phosphorylates AKT and CREB and increases BDNF level in human MSCs exposed to H2O2. The changes of these proteins are possibly relevant to the elevation of agmatine. Collectively, our data demonstrate that the overexpression of human ADC stimulates pro-survival factors to protect human MSCs against H2O2 toxicity. In conclusion, the present fndings support that ADC can enhancethe survival of MSCs against hostile environment of host tissues.

Original languageEnglish
Pages (from-to)366-373
Number of pages8
JournalJournal of Korean medical science
Volume28
Issue number3
DOIs
Publication statusPublished - 2013 Mar 1

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All Science Journal Classification (ASJC) codes

  • Medicine(all)

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