Overexpression of LIM Kinase 1 Renders Resistance to Apoptosis in PC12 Cells by Inhibition of Caspase Activation

E. Yang, H. Kim, Jongeun Lee, J. S. Shin, H. Yoon, S. J. Kim, I. H. Choi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

LIM kinases (LIMKs) regulate actin polymerization by phosphorylating cofilin and are predominantly expressed in neural tissue. In this study, the effect of LIMK1 overexpression in PC12 cell apoptosis was investigated. PC12 cells overexpressing the wild-type LIMK1 were more resistant to serum-withdrawal-induced cell death and the level of caspase 3 activation in these cells was lower than in the control PC12 cells or than in the PC12 cells expressing a mutant LIMK1 lacking the kinase domain. The inhibition of JNK activation was observed in the PC12 cells overexpressing the wild-type LIMK1 after serum withdrawal. These results suggest that the LIMK1 might allow resistance to apoptosis in PC12 cells by inhibiting JNK activation.

Original languageEnglish
Pages (from-to)181-192
Number of pages12
JournalCellular and Molecular Neurobiology
Volume24
Issue number2
DOIs
Publication statusPublished - 2004 Apr 1

Fingerprint

Lim Kinases
PC12 Cells
Caspases
Apoptosis
Actin Depolymerizing Factors
Serum
Caspase 3
Polymerization
Actins
Cell Death
Phosphotransferases

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Yang, E. ; Kim, H. ; Lee, Jongeun ; Shin, J. S. ; Yoon, H. ; Kim, S. J. ; Choi, I. H. / Overexpression of LIM Kinase 1 Renders Resistance to Apoptosis in PC12 Cells by Inhibition of Caspase Activation. In: Cellular and Molecular Neurobiology. 2004 ; Vol. 24, No. 2. pp. 181-192.
@article{b5954954bda24a46a972f1a2e5c0a0d8,
title = "Overexpression of LIM Kinase 1 Renders Resistance to Apoptosis in PC12 Cells by Inhibition of Caspase Activation",
abstract = "LIM kinases (LIMKs) regulate actin polymerization by phosphorylating cofilin and are predominantly expressed in neural tissue. In this study, the effect of LIMK1 overexpression in PC12 cell apoptosis was investigated. PC12 cells overexpressing the wild-type LIMK1 were more resistant to serum-withdrawal-induced cell death and the level of caspase 3 activation in these cells was lower than in the control PC12 cells or than in the PC12 cells expressing a mutant LIMK1 lacking the kinase domain. The inhibition of JNK activation was observed in the PC12 cells overexpressing the wild-type LIMK1 after serum withdrawal. These results suggest that the LIMK1 might allow resistance to apoptosis in PC12 cells by inhibiting JNK activation.",
author = "E. Yang and H. Kim and Jongeun Lee and Shin, {J. S.} and H. Yoon and Kim, {S. J.} and Choi, {I. H.}",
year = "2004",
month = "4",
day = "1",
doi = "10.1023/B:CEMN.0000018615.84358.33",
language = "English",
volume = "24",
pages = "181--192",
journal = "Cellular and Molecular Neurobiology",
issn = "0272-4340",
publisher = "Springer New York",
number = "2",

}

Overexpression of LIM Kinase 1 Renders Resistance to Apoptosis in PC12 Cells by Inhibition of Caspase Activation. / Yang, E.; Kim, H.; Lee, Jongeun; Shin, J. S.; Yoon, H.; Kim, S. J.; Choi, I. H.

In: Cellular and Molecular Neurobiology, Vol. 24, No. 2, 01.04.2004, p. 181-192.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Overexpression of LIM Kinase 1 Renders Resistance to Apoptosis in PC12 Cells by Inhibition of Caspase Activation

AU - Yang, E.

AU - Kim, H.

AU - Lee, Jongeun

AU - Shin, J. S.

AU - Yoon, H.

AU - Kim, S. J.

AU - Choi, I. H.

PY - 2004/4/1

Y1 - 2004/4/1

N2 - LIM kinases (LIMKs) regulate actin polymerization by phosphorylating cofilin and are predominantly expressed in neural tissue. In this study, the effect of LIMK1 overexpression in PC12 cell apoptosis was investigated. PC12 cells overexpressing the wild-type LIMK1 were more resistant to serum-withdrawal-induced cell death and the level of caspase 3 activation in these cells was lower than in the control PC12 cells or than in the PC12 cells expressing a mutant LIMK1 lacking the kinase domain. The inhibition of JNK activation was observed in the PC12 cells overexpressing the wild-type LIMK1 after serum withdrawal. These results suggest that the LIMK1 might allow resistance to apoptosis in PC12 cells by inhibiting JNK activation.

AB - LIM kinases (LIMKs) regulate actin polymerization by phosphorylating cofilin and are predominantly expressed in neural tissue. In this study, the effect of LIMK1 overexpression in PC12 cell apoptosis was investigated. PC12 cells overexpressing the wild-type LIMK1 were more resistant to serum-withdrawal-induced cell death and the level of caspase 3 activation in these cells was lower than in the control PC12 cells or than in the PC12 cells expressing a mutant LIMK1 lacking the kinase domain. The inhibition of JNK activation was observed in the PC12 cells overexpressing the wild-type LIMK1 after serum withdrawal. These results suggest that the LIMK1 might allow resistance to apoptosis in PC12 cells by inhibiting JNK activation.

UR - http://www.scopus.com/inward/record.url?scp=1842783552&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842783552&partnerID=8YFLogxK

U2 - 10.1023/B:CEMN.0000018615.84358.33

DO - 10.1023/B:CEMN.0000018615.84358.33

M3 - Article

VL - 24

SP - 181

EP - 192

JO - Cellular and Molecular Neurobiology

JF - Cellular and Molecular Neurobiology

SN - 0272-4340

IS - 2

ER -