Overproduction of cellulose in Acetobacter xylinum KCCM 10100 defective in GDP-mannosyltransferase

Sang Tae Park, Eungbin Kim, Young Min Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

GDP-mannosyltransferase (GMT) is an enzyme responsible for the addition of a mannose to glucose (α[1→3]) during biosynthesis of the water-soluble branched polysaccharide acetan in Acetobacter species. In an effort to obtain a cellulose-overproducing bacterium, a mutant defective in GMT of Acetobacter xylinum KCCM 10100 was constructed by single crossover homologous recombination using part of the aceA gene encoding GMT amplified by polymerase chain reaction. The GMT-disrupted mutant produced 23% more cellulose, but 16% less water-soluble polysaccharide than those of the wild-type strain. Analysis of the sugar composition by gel permeation chromatography revealed that water-soluble polysaccharides produced by the GMT-defective mutant contained no mannose molecule.

Original languageEnglish
Pages (from-to)961-964
Number of pages4
JournalJournal of microbiology and biotechnology
Volume16
Issue number6
Publication statusPublished - 2006 Jun 1

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Gluconacetobacter xylinus
Cellulose
Polysaccharides
Mannose
Water
Acetobacter
Homologous Recombination
Gel Chromatography
GDP-mannosyltransferase
Bacteria
Glucose
Polymerase Chain Reaction
Enzymes
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "GDP-mannosyltransferase (GMT) is an enzyme responsible for the addition of a mannose to glucose (α[1→3]) during biosynthesis of the water-soluble branched polysaccharide acetan in Acetobacter species. In an effort to obtain a cellulose-overproducing bacterium, a mutant defective in GMT of Acetobacter xylinum KCCM 10100 was constructed by single crossover homologous recombination using part of the aceA gene encoding GMT amplified by polymerase chain reaction. The GMT-disrupted mutant produced 23{\%} more cellulose, but 16{\%} less water-soluble polysaccharide than those of the wild-type strain. Analysis of the sugar composition by gel permeation chromatography revealed that water-soluble polysaccharides produced by the GMT-defective mutant contained no mannose molecule.",
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Overproduction of cellulose in Acetobacter xylinum KCCM 10100 defective in GDP-mannosyltransferase. / Park, Sang Tae; Kim, Eungbin; Kim, Young Min.

In: Journal of microbiology and biotechnology, Vol. 16, No. 6, 01.06.2006, p. 961-964.

Research output: Contribution to journalArticle

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