Oxidation of DNA Bases Influenced by the Presence of Other Bases

Madeline ShuhuaGoh, Martin Pumera

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Electrochemical detection of DNA is a highly important topic. Here we show that the electrochemical responses of one DNA base (guanine, adenine, cytosine or thymine), in terms of oxidation potential, current intensity, peak width and resolution can be highly influenced by the presence of other DNA bases at electrochemically reduced graphene oxide (ER-GO) as well as standard glassy carbon electrode. We have observed that the effects were more significant for adenine base on ER-GO and cytosine base on glassy carbon (GC) electrode. Differences in responses were generally low in a mixture of four different DNA bases but interestingly, deviations become significantly larger when only one or two other bases were present. Our findings are of paramount importance for future developments in DNA detection and analysis since individual DNA bases are not present in isolation in nature or in typical biosensing systems.

Original languageEnglish
Pages (from-to)1147-1152
Number of pages6
JournalElectroanalysis
Volume24
Issue number5
DOIs
Publication statusPublished - 2012 May 1

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Electrochemistry

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