P22-Based Challenge Phage Constructs to Study DNA-Protein Interactions between the σ54-Dependent Promoter, dctA, and Its Transcriptional Regulators

Eungbin Kim, Daeyou Kim, Joon Haeng Lee

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1 Citation (Scopus)


A challenge phage system was used to study the DNA-protein interaction between C4-dicarboylic acid transport protein D (DCTD) or σ54, and a σ54-dependent promoter, dctAp. R. meliloti dctA promoter regulatory region replaced the Omnt site on the phage. S. typhimurium strains overproducing either DCTD or σ54 directed this challenge phage towards lysogeny, indicating that DCTD or Eσ54 recognized the dctA promoter on the phage and repressed transcription of the ant gene. These challenge phage constructs will be useful for examining interactions between DCTD (or σ54) and the dctA promoter region.

Original languageEnglish
Pages (from-to)176-179
Number of pages4
JournalJournal of Microbiology
Issue number3
Publication statusPublished - 2000 Dec 1


All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

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