P22-Based Challenge Phage Constructs to Study DNA-Protein Interactions between the σ54-Dependent Promoter, dctA, and Its Transcriptional Regulators

Eungbin Kim; Daeyou Kim; Joon Haeng Lee

P22-Based Challenge Phage Constructs to Study DNA-Protein Interactions between the σ⁵⁴-Dependent Promoter, dctA, and Its Transcriptional Regulators

Eungbin Kim, Daeyou Kim, Joon Haeng Lee

Department of Systems Biology

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A challenge phage system was used to study the DNA-protein interaction between C₄-dicarboylic acid transport protein D (DCTD) or σ⁵⁴, and a σ⁵⁴-dependent promoter, dctAp. R. meliloti dctA promoter regulatory region replaced the O_mnt site on the phage. S. typhimurium strains overproducing either DCTD or σ⁵⁴ directed this challenge phage towards lysogeny, indicating that DCTD or Eσ⁵⁴ recognized the dctA promoter on the phage and repressed transcription of the ant gene. These challenge phage constructs will be useful for examining interactions between DCTD (or σ⁵⁴) and the dctA promoter region.

Original language	English
Pages (from-to)	176-179
Number of pages	4
Journal	Journal of Microbiology
Volume	38
Issue number	3
Publication status	Published - 2000

All Science Journal Classification (ASJC) codes

Microbiology
Applied Microbiology and Biotechnology

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abstract = "A challenge phage system was used to study the DNA-protein interaction between C4-dicarboylic acid transport protein D (DCTD) or σ54, and a σ54-dependent promoter, dctAp. R. meliloti dctA promoter regulatory region replaced the Omnt site on the phage. S. typhimurium strains overproducing either DCTD or σ54 directed this challenge phage towards lysogeny, indicating that DCTD or Eσ54 recognized the dctA promoter on the phage and repressed transcription of the ant gene. These challenge phage constructs will be useful for examining interactions between DCTD (or σ54) and the dctA promoter region.",

author = "Eungbin Kim and Daeyou Kim and Lee, {Joon Haeng}",

year = "2000",

language = "English",

volume = "38",

pages = "176--179",

journal = "Journal of Microbiology",

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AU - Kim, Daeyou

AU - Lee, Joon Haeng

PY - 2000

Y1 - 2000

N2 - A challenge phage system was used to study the DNA-protein interaction between C4-dicarboylic acid transport protein D (DCTD) or σ54, and a σ54-dependent promoter, dctAp. R. meliloti dctA promoter regulatory region replaced the Omnt site on the phage. S. typhimurium strains overproducing either DCTD or σ54 directed this challenge phage towards lysogeny, indicating that DCTD or Eσ54 recognized the dctA promoter on the phage and repressed transcription of the ant gene. These challenge phage constructs will be useful for examining interactions between DCTD (or σ54) and the dctA promoter region.

AB - A challenge phage system was used to study the DNA-protein interaction between C4-dicarboylic acid transport protein D (DCTD) or σ54, and a σ54-dependent promoter, dctAp. R. meliloti dctA promoter regulatory region replaced the Omnt site on the phage. S. typhimurium strains overproducing either DCTD or σ54 directed this challenge phage towards lysogeny, indicating that DCTD or Eσ54 recognized the dctA promoter on the phage and repressed transcription of the ant gene. These challenge phage constructs will be useful for examining interactions between DCTD (or σ54) and the dctA promoter region.

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