Abstract
To study interactions between C4-dicarboxylic acid transport protein D and Eσ54 in the dctA promoter regulatory region, we used the challenge phage system. An ant'-'lac fusion was recombined onto the challenge phage, and this ant'-'lac fusion along with Pant and the R. meliloti dctA promoter regulatory region were cloned onto a plasmid. The plasmid bearing the ant'-'lac fusion was used as a reporter plasmid in a coupled transcription-translation system. Addition of purified σ54 to the coupled system specifically repressed transcription of the plasmid-borne ant'-'lac fusion. When DCTD was added along with σ54 to the coupled system, transcription of the ant'-'lac fusion was even further repressed, suggesting that DCTD may stabilize closed complexes between Eσ54 and the dctA promoter.
Original language | English |
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Pages (from-to) | 205-210 |
Number of pages | 6 |
Journal | Journal of Microbiology |
Volume | 40 |
Issue number | 3 |
Publication status | Published - 2002 Sep |
All Science Journal Classification (ASJC) codes
- Microbiology
- Applied Microbiology and Biotechnology