To study interactions between C4-dicarboxylic acid transport protein D and Eσ54 in the dctA promoter regulatory region, we used the challenge phage system. An ant'-'lac fusion was recombined onto the challenge phage, and this ant'-'lac fusion along with Pant and the R. meliloti dctA promoter regulatory region were cloned onto a plasmid. The plasmid bearing the ant'-'lac fusion was used as a reporter plasmid in a coupled transcription-translation system. Addition of purified σ54 to the coupled system specifically repressed transcription of the plasmid-borne ant'-'lac fusion. When DCTD was added along with σ54 to the coupled system, transcription of the ant'-'lac fusion was even further repressed, suggesting that DCTD may stabilize closed complexes between Eσ54 and the dctA promoter.
|Number of pages||6|
|Journal||Journal of Microbiology|
|Publication status||Published - 2002 Sep 1|
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology