Abstract
Klebsiella pneumoniae is responsible for numerous infections caused in hospitals, leading to mortality and morbidity. It has been evolving as a multi-drug resistant pathogen, acquiring multiple resistances such as such as horizontal gene transfer, transposon-mediated insertions or change in outer membrane permeability. Therefore, constant efforts are being carried out to control the infections using various antibiotic therapies. Considering the severity of the acquired resistance, we developed a panel of strains of K. pneumoniae expressing different resistance profiles such as high-level penicillinase and AmpC production, extended spectrum beta-lactamases and carbapenemases. Bacterial strains expressing different resistance phenotypes were collected and examined for resistance genes, mutations and porin alterations contributing to the detected phenotypes. Using the Massive parallel sequencing (MPS) technology we have constructed and genotypically characterized the panel strains to elucidate the multidrug resistance. These panel strains can be used in the clinical laboratory as standard reference strains. In addition, these strains could be significant in the field of pharmaceuticals for the antibiotic drug testing to verify its efficiency on pathogens expressing various resistances.
| Original language | English |
|---|---|
| Article number | e2896 |
| Journal | PeerJ |
| Volume | 2017 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2017 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
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Panel strain of Klebsiella pneumoniae for beta-lactam antibiotic evaluation : Their phenotypic and genotypic characterization. / Dsouza, Roshan; Pinto, Naina Adren; Hwang, In Sik; Cho, Young Lag; Yong, Dongeun; Choi, Jongrak; Lee, Kyungwon; Chong, Yunsop.
In: PeerJ, Vol. 2017, No. 1, e2896, 01.01.2017.Research output: Contribution to journal › Article
TY - JOUR
T1 - Panel strain of Klebsiella pneumoniae for beta-lactam antibiotic evaluation
T2 - Their phenotypic and genotypic characterization
AU - Dsouza, Roshan
AU - Pinto, Naina Adren
AU - Hwang, In Sik
AU - Cho, Young Lag
AU - Yong, Dongeun
AU - Choi, Jongrak
AU - Lee, Kyungwon
AU - Chong, Yunsop
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Klebsiella pneumoniae is responsible for numerous infections caused in hospitals, leading to mortality and morbidity. It has been evolving as a multi-drug resistant pathogen, acquiring multiple resistances such as such as horizontal gene transfer, transposon-mediated insertions or change in outer membrane permeability. Therefore, constant efforts are being carried out to control the infections using various antibiotic therapies. Considering the severity of the acquired resistance, we developed a panel of strains of K. pneumoniae expressing different resistance profiles such as high-level penicillinase and AmpC production, extended spectrum beta-lactamases and carbapenemases. Bacterial strains expressing different resistance phenotypes were collected and examined for resistance genes, mutations and porin alterations contributing to the detected phenotypes. Using the Massive parallel sequencing (MPS) technology we have constructed and genotypically characterized the panel strains to elucidate the multidrug resistance. These panel strains can be used in the clinical laboratory as standard reference strains. In addition, these strains could be significant in the field of pharmaceuticals for the antibiotic drug testing to verify its efficiency on pathogens expressing various resistances.
AB - Klebsiella pneumoniae is responsible for numerous infections caused in hospitals, leading to mortality and morbidity. It has been evolving as a multi-drug resistant pathogen, acquiring multiple resistances such as such as horizontal gene transfer, transposon-mediated insertions or change in outer membrane permeability. Therefore, constant efforts are being carried out to control the infections using various antibiotic therapies. Considering the severity of the acquired resistance, we developed a panel of strains of K. pneumoniae expressing different resistance profiles such as high-level penicillinase and AmpC production, extended spectrum beta-lactamases and carbapenemases. Bacterial strains expressing different resistance phenotypes were collected and examined for resistance genes, mutations and porin alterations contributing to the detected phenotypes. Using the Massive parallel sequencing (MPS) technology we have constructed and genotypically characterized the panel strains to elucidate the multidrug resistance. These panel strains can be used in the clinical laboratory as standard reference strains. In addition, these strains could be significant in the field of pharmaceuticals for the antibiotic drug testing to verify its efficiency on pathogens expressing various resistances.
UR - http://www.scopus.com/inward/record.url?scp=85011299389&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85011299389&partnerID=8YFLogxK
U2 - 10.7717/peerj.2896
DO - 10.7717/peerj.2896
M3 - Article
AN - SCOPUS:85011299389
VL - 2017
JO - PeerJ
JF - PeerJ
SN - 2167-8359
IS - 1
M1 - e2896
ER -