Panel strain of Klebsiella pneumoniae for beta-lactam antibiotic evaluation: Their phenotypic and genotypic characterization

Roshan Dsouza, Naina Adren Pinto, In Sik Hwang, Young Lag Cho, Dongeun Yong, Jongrak Choi, Kyungwon Lee, Yunsop Chong

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Klebsiella pneumoniae is responsible for numerous infections caused in hospitals, leading to mortality and morbidity. It has been evolving as a multi-drug resistant pathogen, acquiring multiple resistances such as such as horizontal gene transfer, transposon-mediated insertions or change in outer membrane permeability. Therefore, constant efforts are being carried out to control the infections using various antibiotic therapies. Considering the severity of the acquired resistance, we developed a panel of strains of K. pneumoniae expressing different resistance profiles such as high-level penicillinase and AmpC production, extended spectrum beta-lactamases and carbapenemases. Bacterial strains expressing different resistance phenotypes were collected and examined for resistance genes, mutations and porin alterations contributing to the detected phenotypes. Using the Massive parallel sequencing (MPS) technology we have constructed and genotypically characterized the panel strains to elucidate the multidrug resistance. These panel strains can be used in the clinical laboratory as standard reference strains. In addition, these strains could be significant in the field of pharmaceuticals for the antibiotic drug testing to verify its efficiency on pathogens expressing various resistances.

Original languageEnglish
Article numbere2896
JournalPeerJ
Volume2017
Issue number1
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

beta-lactam antibiotics
Klebsiella pneumoniae
beta-Lactams
Anti-Bacterial Agents
Pharmaceutical Preparations
Phenotype
Penicillinase
Pathogens
Horizontal Gene Transfer
Porins
beta-lactamase
Multiple Drug Resistance
beta-Lactamases
Infection Control
drugs
Clinical laboratories
Permeability
Gene transfer
antibiotics
Technology

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Dsouza, Roshan ; Pinto, Naina Adren ; Hwang, In Sik ; Cho, Young Lag ; Yong, Dongeun ; Choi, Jongrak ; Lee, Kyungwon ; Chong, Yunsop. / Panel strain of Klebsiella pneumoniae for beta-lactam antibiotic evaluation : Their phenotypic and genotypic characterization. In: PeerJ. 2017 ; Vol. 2017, No. 1.
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Panel strain of Klebsiella pneumoniae for beta-lactam antibiotic evaluation : Their phenotypic and genotypic characterization. / Dsouza, Roshan; Pinto, Naina Adren; Hwang, In Sik; Cho, Young Lag; Yong, Dongeun; Choi, Jongrak; Lee, Kyungwon; Chong, Yunsop.

In: PeerJ, Vol. 2017, No. 1, e2896, 01.01.2017.

Research output: Contribution to journalArticle

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AU - Dsouza, Roshan

AU - Pinto, Naina Adren

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AU - Cho, Young Lag

AU - Yong, Dongeun

AU - Choi, Jongrak

AU - Lee, Kyungwon

AU - Chong, Yunsop

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