Paper-based bioactive scaffolds for stem cell-mediated bone tissue engineering

Hyun Ji Park, Seung Jung Yu, Kisuk Yang, Yoonhee Jin, Ann Na Cho, Jin Kim, Bora Lee, Hee Seok Yang, Sung Gap Im, Seung-Woo Cho

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Bioactive, functional scaffolds are required to improve the regenerative potential of stem cells for tissue reconstruction and functional recovery of damaged tissues. Here, we report a paper-based bioactive scaffold platform for stem cell culture and transplantation for bone reconstruction. The paper scaffolds are surface-engineered by an initiated chemical vapor deposition process for serial coating of a water-repellent and cell-adhesive polymer film, which ensures the long-term stability in cell culture medium and induces efficient cell attachment. The prepared paper scaffolds are compatible with general stem cell culture and manipulation techniques. An optimal paper type is found to provide structural, physical, and mechanical cues to enhance the osteogenic differentiation of human adipose-derived stem cells (hADSCs). A bioactive paper scaffold significantly enhances in vivo bone regeneration of hADSCs in a critical-sized calvarial bone defect. Stacking the paper scaffolds with osteogenically differentiated hADSCs and human endothelial cells resulted in vascularized bone formation in vivo. Our study suggests that paper possesses great potential as a bioactive, functional, and cost-effective scaffold platform for stem cell-mediated bone tissue engineering. To the best of our knowledge, this is the first study reporting the feasibility of a paper material for stem cell application to repair tissue defects.

Original languageEnglish
Pages (from-to)9811-9823
Number of pages13
JournalBiomaterials
Volume35
Issue number37
DOIs
Publication statusPublished - 2014 Dec 1

Fingerprint

Tissue Engineering
Scaffolds (biology)
Stem cells
Tissue engineering
Bone
Stem Cells
Bone and Bones
Cell culture
Scaffolds
Cell Culture Techniques
Tissue
Bone Regeneration
Feasibility Studies
Defects
Stem Cell Transplantation
Osteogenesis
Endothelial cells
Adhesives
Cues
Culture Media

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Park, Hyun Ji ; Yu, Seung Jung ; Yang, Kisuk ; Jin, Yoonhee ; Cho, Ann Na ; Kim, Jin ; Lee, Bora ; Yang, Hee Seok ; Im, Sung Gap ; Cho, Seung-Woo. / Paper-based bioactive scaffolds for stem cell-mediated bone tissue engineering. In: Biomaterials. 2014 ; Vol. 35, No. 37. pp. 9811-9823.
@article{021e03f0db7441e98c889d78abc4fd4f,
title = "Paper-based bioactive scaffolds for stem cell-mediated bone tissue engineering",
abstract = "Bioactive, functional scaffolds are required to improve the regenerative potential of stem cells for tissue reconstruction and functional recovery of damaged tissues. Here, we report a paper-based bioactive scaffold platform for stem cell culture and transplantation for bone reconstruction. The paper scaffolds are surface-engineered by an initiated chemical vapor deposition process for serial coating of a water-repellent and cell-adhesive polymer film, which ensures the long-term stability in cell culture medium and induces efficient cell attachment. The prepared paper scaffolds are compatible with general stem cell culture and manipulation techniques. An optimal paper type is found to provide structural, physical, and mechanical cues to enhance the osteogenic differentiation of human adipose-derived stem cells (hADSCs). A bioactive paper scaffold significantly enhances in vivo bone regeneration of hADSCs in a critical-sized calvarial bone defect. Stacking the paper scaffolds with osteogenically differentiated hADSCs and human endothelial cells resulted in vascularized bone formation in vivo. Our study suggests that paper possesses great potential as a bioactive, functional, and cost-effective scaffold platform for stem cell-mediated bone tissue engineering. To the best of our knowledge, this is the first study reporting the feasibility of a paper material for stem cell application to repair tissue defects.",
author = "Park, {Hyun Ji} and Yu, {Seung Jung} and Kisuk Yang and Yoonhee Jin and Cho, {Ann Na} and Jin Kim and Bora Lee and Yang, {Hee Seok} and Im, {Sung Gap} and Seung-Woo Cho",
year = "2014",
month = "12",
day = "1",
doi = "10.1016/j.biomaterials.2014.09.002",
language = "English",
volume = "35",
pages = "9811--9823",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "37",

}

Park, HJ, Yu, SJ, Yang, K, Jin, Y, Cho, AN, Kim, J, Lee, B, Yang, HS, Im, SG & Cho, S-W 2014, 'Paper-based bioactive scaffolds for stem cell-mediated bone tissue engineering', Biomaterials, vol. 35, no. 37, pp. 9811-9823. https://doi.org/10.1016/j.biomaterials.2014.09.002

Paper-based bioactive scaffolds for stem cell-mediated bone tissue engineering. / Park, Hyun Ji; Yu, Seung Jung; Yang, Kisuk; Jin, Yoonhee; Cho, Ann Na; Kim, Jin; Lee, Bora; Yang, Hee Seok; Im, Sung Gap; Cho, Seung-Woo.

In: Biomaterials, Vol. 35, No. 37, 01.12.2014, p. 9811-9823.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Paper-based bioactive scaffolds for stem cell-mediated bone tissue engineering

AU - Park, Hyun Ji

AU - Yu, Seung Jung

AU - Yang, Kisuk

AU - Jin, Yoonhee

AU - Cho, Ann Na

AU - Kim, Jin

AU - Lee, Bora

AU - Yang, Hee Seok

AU - Im, Sung Gap

AU - Cho, Seung-Woo

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Bioactive, functional scaffolds are required to improve the regenerative potential of stem cells for tissue reconstruction and functional recovery of damaged tissues. Here, we report a paper-based bioactive scaffold platform for stem cell culture and transplantation for bone reconstruction. The paper scaffolds are surface-engineered by an initiated chemical vapor deposition process for serial coating of a water-repellent and cell-adhesive polymer film, which ensures the long-term stability in cell culture medium and induces efficient cell attachment. The prepared paper scaffolds are compatible with general stem cell culture and manipulation techniques. An optimal paper type is found to provide structural, physical, and mechanical cues to enhance the osteogenic differentiation of human adipose-derived stem cells (hADSCs). A bioactive paper scaffold significantly enhances in vivo bone regeneration of hADSCs in a critical-sized calvarial bone defect. Stacking the paper scaffolds with osteogenically differentiated hADSCs and human endothelial cells resulted in vascularized bone formation in vivo. Our study suggests that paper possesses great potential as a bioactive, functional, and cost-effective scaffold platform for stem cell-mediated bone tissue engineering. To the best of our knowledge, this is the first study reporting the feasibility of a paper material for stem cell application to repair tissue defects.

AB - Bioactive, functional scaffolds are required to improve the regenerative potential of stem cells for tissue reconstruction and functional recovery of damaged tissues. Here, we report a paper-based bioactive scaffold platform for stem cell culture and transplantation for bone reconstruction. The paper scaffolds are surface-engineered by an initiated chemical vapor deposition process for serial coating of a water-repellent and cell-adhesive polymer film, which ensures the long-term stability in cell culture medium and induces efficient cell attachment. The prepared paper scaffolds are compatible with general stem cell culture and manipulation techniques. An optimal paper type is found to provide structural, physical, and mechanical cues to enhance the osteogenic differentiation of human adipose-derived stem cells (hADSCs). A bioactive paper scaffold significantly enhances in vivo bone regeneration of hADSCs in a critical-sized calvarial bone defect. Stacking the paper scaffolds with osteogenically differentiated hADSCs and human endothelial cells resulted in vascularized bone formation in vivo. Our study suggests that paper possesses great potential as a bioactive, functional, and cost-effective scaffold platform for stem cell-mediated bone tissue engineering. To the best of our knowledge, this is the first study reporting the feasibility of a paper material for stem cell application to repair tissue defects.

UR - http://www.scopus.com/inward/record.url?scp=84907259440&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907259440&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2014.09.002

DO - 10.1016/j.biomaterials.2014.09.002

M3 - Article

VL - 35

SP - 9811

EP - 9823

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 37

ER -