Parkin induces apoptotic cell death in TNF-αtreated cervical cancer cells

Kyunghong Lee, Min Ho Lee, Yeo Wool Kang, Kijong Rhee, Tae Ue Kim, Yoon Suk Kim

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Many malignant tumors become resistant to tumor necrosis factor-alpha (TNF-α)-induced celldeathduring carcinogenesis. In the present study, we examined whether parkin acts as a tumor suppressor inHeLa cells, a human cervical cancer cell line resistant to TNF-α-induced cell death. TNF-a-treatment alone did not affect HeLa cell viability. However, expression of parkin restored TNF-α-induced apoptosis in HeLa cells. Increased cell death was due to the activation of the apoptotic pathway. Expression of parkin in TNF-α-treated HeLa cells stimulated cleavage of the pro-apoptotic proteins caspase-8, -9, -3, -7 and poly ADP ribose polymerase (PARP). In addition, parkin expression resulted in decreased expression of the caspase inhibitory protein, survivin. These results suggest that parkin acts as atumor suppressor in human cervical cancer cells by modulating survivin expression and caspase activity. We propose that this pathway is a novel molecular mechanism by which parkinfunctions as a tumor suppressor.

Original languageEnglish
Pages (from-to)521-525
Number of pages5
JournalBMB reports
Volume45
Issue number9
DOIs
Publication statusPublished - 2012 Jan 1

Fingerprint

Cell death
Uterine Cervical Neoplasms
Cell Death
Tumor Necrosis Factor-alpha
Cells
HeLa Cells
Tumors
Caspases
Neoplasms
Apoptosis Regulatory Proteins
Poly(ADP-ribose) Polymerases
Caspase 9
Caspase 8
Cell Survival
Carcinogenesis
Chemical activation
Apoptosis
Cell Line
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Lee, Kyunghong ; Lee, Min Ho ; Kang, Yeo Wool ; Rhee, Kijong ; Kim, Tae Ue ; Kim, Yoon Suk. / Parkin induces apoptotic cell death in TNF-αtreated cervical cancer cells. In: BMB reports. 2012 ; Vol. 45, No. 9. pp. 521-525.
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Parkin induces apoptotic cell death in TNF-αtreated cervical cancer cells. / Lee, Kyunghong; Lee, Min Ho; Kang, Yeo Wool; Rhee, Kijong; Kim, Tae Ue; Kim, Yoon Suk.

In: BMB reports, Vol. 45, No. 9, 01.01.2012, p. 521-525.

Research output: Contribution to journalArticle

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