Partial characterization of phosphotriesterase activity from the earthworm, Eisenia andrei

Myung Sik Lee, Sung Jin Cho, Eun Sik Tak, Ki Seok Koh, Jong Kil Choo, Hee Woo Park, Eungbin Kim, Youngeun Na, Soon Cheol Park

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Phosphotriesterase (PTE) receives attention because it seems to be associated with the detoxification of organophosphorous pesticides and organophosphate resistance mechanism. In order to understand the biodegradation of phosphotriester pesticides and its significance in the earthworm, a major non-target animal of pesticides, selected properties of phosphotriesterase activity derived from the crude extract of Eisenia andrei were investigated. PTE activity appeared to be primarily localized in intestinal tissues. The highest level of PTE activity was found in epithelial tissue. The native molecular weight of earthwom PTE was 260 kDa and the isoelectric point was approximately 4. The optimal pH was approximately 9. The earthwonn PTE had a substrate affinity for paraoxon with Km value in the millimolar range. The presence of EGTA and EDTA completely abolished rite activity and replacement of Ca2+ ion restored activity to greater than 95%, suggesting that Ca2+ ion is essential to maintain the activity.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalInternational Biodeterioration and Biodegradation
Volume47
Issue number1
DOIs
Publication statusPublished - 2001 Mar 14

Fingerprint

Phosphoric Triester Hydrolases
Oligochaeta
Pesticides
earthworm
pesticide
Tissue
Detoxification
ion
organophosphate
Ethylenediaminetetraacetic acid
Ions
detoxification
Biodegradation
EDTA
biodegradation
Animals
replacement
Molecular weight
Paraoxon
substrate

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biomaterials
  • Waste Management and Disposal

Cite this

Sik Lee, M., Jin Cho, S., Sik Tak, E., Seok Koh, K., Kil Choo, J., Woo Park, H., ... Cheol Park, S. (2001). Partial characterization of phosphotriesterase activity from the earthworm, Eisenia andrei. International Biodeterioration and Biodegradation, 47(1), 1-5. https://doi.org/10.1016/S0964-8305(00)00098-6
Sik Lee, Myung ; Jin Cho, Sung ; Sik Tak, Eun ; Seok Koh, Ki ; Kil Choo, Jong ; Woo Park, Hee ; Kim, Eungbin ; Na, Youngeun ; Cheol Park, Soon. / Partial characterization of phosphotriesterase activity from the earthworm, Eisenia andrei. In: International Biodeterioration and Biodegradation. 2001 ; Vol. 47, No. 1. pp. 1-5.
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Sik Lee, M, Jin Cho, S, Sik Tak, E, Seok Koh, K, Kil Choo, J, Woo Park, H, Kim, E, Na, Y & Cheol Park, S 2001, 'Partial characterization of phosphotriesterase activity from the earthworm, Eisenia andrei', International Biodeterioration and Biodegradation, vol. 47, no. 1, pp. 1-5. https://doi.org/10.1016/S0964-8305(00)00098-6

Partial characterization of phosphotriesterase activity from the earthworm, Eisenia andrei. / Sik Lee, Myung; Jin Cho, Sung; Sik Tak, Eun; Seok Koh, Ki; Kil Choo, Jong; Woo Park, Hee; Kim, Eungbin; Na, Youngeun; Cheol Park, Soon.

In: International Biodeterioration and Biodegradation, Vol. 47, No. 1, 14.03.2001, p. 1-5.

Research output: Contribution to journalArticle

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AU - Woo Park, Hee

AU - Kim, Eungbin

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AU - Cheol Park, Soon

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