Partial characterization of phosphotriesterase activity from the earthworm, Eisenia andrei

Myung Sik Lee, Sung Jin Cho, Eun Sik Tak, Ki Seok Koh, Jong Kil Choo, Hee Woo Park, Eungbin Kim, Youngeun Na, Soon Cheol Park

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Phosphotriesterase (PTE) receives attention because it seems to be associated with the detoxification of organophosphorous pesticides and organophosphate resistance mechanism. In order to understand the biodegradation of phosphotriester pesticides and its significance in the earthworm, a major non-target animal of pesticides, selected properties of phosphotriesterase activity derived from the crude extract of Eisenia andrei were investigated. PTE activity appeared to be primarily localized in intestinal tissues. The highest level of PTE activity was found in epithelial tissue. The native molecular weight of earthwom PTE was 260 kDa and the isoelectric point was approximately 4. The optimal pH was approximately 9. The earthwonn PTE had a substrate affinity for paraoxon with Km value in the millimolar range. The presence of EGTA and EDTA completely abolished rite activity and replacement of Ca2+ ion restored activity to greater than 95%, suggesting that Ca2+ ion is essential to maintain the activity.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalInternational Biodeterioration and Biodegradation
Volume47
Issue number1
DOIs
Publication statusPublished - 2001

Bibliographical note

Funding Information:
This paper was supported by NON DIRECTED RESEARCH FUND, Korea Research Foundation, 1996.

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biomaterials
  • Waste Management and Disposal

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