Partial characterization of phosphotriesterase activity from the earthworm, Eisenia andrei

Myung Sik Lee; Sung Jin Cho; Eun Sik Tak; Ki Seok Koh; Jong Kil Choo; Hee Woo Park; Eungbin Kim; Youngeun Na; Soon Cheol Park

doi:10.1016/S0964-8305(00)00098-6

Partial characterization of phosphotriesterase activity from the earthworm, Eisenia andrei

Myung Sik Lee, Sung Jin Cho, Eun Sik Tak, Ki Seok Koh, Jong Kil Choo, Hee Woo Park, Eungbin Kim, Youngeun Na, Soon Cheol Park

Department of Systems Biology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Phosphotriesterase (PTE) receives attention because it seems to be associated with the detoxification of organophosphorous pesticides and organophosphate resistance mechanism. In order to understand the biodegradation of phosphotriester pesticides and its significance in the earthworm, a major non-target animal of pesticides, selected properties of phosphotriesterase activity derived from the crude extract of Eisenia andrei were investigated. PTE activity appeared to be primarily localized in intestinal tissues. The highest level of PTE activity was found in epithelial tissue. The native molecular weight of earthwom PTE was 260 kDa and the isoelectric point was approximately 4. The optimal pH was approximately 9. The earthwonn PTE had a substrate affinity for paraoxon with K_m value in the millimolar range. The presence of EGTA and EDTA completely abolished rite activity and replacement of Ca²⁺ ion restored activity to greater than 95%, suggesting that Ca²⁺ ion is essential to maintain the activity.

Original language	English
Pages (from-to)	1-5
Number of pages	5
Journal	International Biodeterioration and Biodegradation
Volume	47
Issue number	1
DOIs	https://doi.org/10.1016/S0964-8305(00)00098-6
Publication status	Published - 2001

Bibliographical note

Funding Information:
This paper was supported by NON DIRECTED RESEARCH FUND, Korea Research Foundation, 1996.

All Science Journal Classification (ASJC) codes

Microbiology
Biomaterials
Waste Management and Disposal

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10.1016/S0964-8305(00)00098-6

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title = "Partial characterization of phosphotriesterase activity from the earthworm, Eisenia andrei",

abstract = "Phosphotriesterase (PTE) receives attention because it seems to be associated with the detoxification of organophosphorous pesticides and organophosphate resistance mechanism. In order to understand the biodegradation of phosphotriester pesticides and its significance in the earthworm, a major non-target animal of pesticides, selected properties of phosphotriesterase activity derived from the crude extract of Eisenia andrei were investigated. PTE activity appeared to be primarily localized in intestinal tissues. The highest level of PTE activity was found in epithelial tissue. The native molecular weight of earthwom PTE was 260 kDa and the isoelectric point was approximately 4. The optimal pH was approximately 9. The earthwonn PTE had a substrate affinity for paraoxon with Km value in the millimolar range. The presence of EGTA and EDTA completely abolished rite activity and replacement of Ca2+ ion restored activity to greater than 95%, suggesting that Ca2+ ion is essential to maintain the activity.",

author = "{Sik Lee}, Myung and {Jin Cho}, Sung and {Sik Tak}, Eun and {Seok Koh}, Ki and {Kil Choo}, Jong and {Woo Park}, Hee and Eungbin Kim and Youngeun Na and {Cheol Park}, Soon",

note = "Funding Information: This paper was supported by NON DIRECTED RESEARCH FUND, Korea Research Foundation, 1996.",

year = "2001",

doi = "10.1016/S0964-8305(00)00098-6",

language = "English",

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AU - Sik Lee, Myung

AU - Jin Cho, Sung

AU - Sik Tak, Eun

AU - Seok Koh, Ki

AU - Kil Choo, Jong

AU - Woo Park, Hee

AU - Kim, Eungbin

AU - Na, Youngeun

AU - Cheol Park, Soon

N1 - Funding Information: This paper was supported by NON DIRECTED RESEARCH FUND, Korea Research Foundation, 1996.

PY - 2001

Y1 - 2001

N2 - Phosphotriesterase (PTE) receives attention because it seems to be associated with the detoxification of organophosphorous pesticides and organophosphate resistance mechanism. In order to understand the biodegradation of phosphotriester pesticides and its significance in the earthworm, a major non-target animal of pesticides, selected properties of phosphotriesterase activity derived from the crude extract of Eisenia andrei were investigated. PTE activity appeared to be primarily localized in intestinal tissues. The highest level of PTE activity was found in epithelial tissue. The native molecular weight of earthwom PTE was 260 kDa and the isoelectric point was approximately 4. The optimal pH was approximately 9. The earthwonn PTE had a substrate affinity for paraoxon with Km value in the millimolar range. The presence of EGTA and EDTA completely abolished rite activity and replacement of Ca2+ ion restored activity to greater than 95%, suggesting that Ca2+ ion is essential to maintain the activity.

AB - Phosphotriesterase (PTE) receives attention because it seems to be associated with the detoxification of organophosphorous pesticides and organophosphate resistance mechanism. In order to understand the biodegradation of phosphotriester pesticides and its significance in the earthworm, a major non-target animal of pesticides, selected properties of phosphotriesterase activity derived from the crude extract of Eisenia andrei were investigated. PTE activity appeared to be primarily localized in intestinal tissues. The highest level of PTE activity was found in epithelial tissue. The native molecular weight of earthwom PTE was 260 kDa and the isoelectric point was approximately 4. The optimal pH was approximately 9. The earthwonn PTE had a substrate affinity for paraoxon with Km value in the millimolar range. The presence of EGTA and EDTA completely abolished rite activity and replacement of Ca2+ ion restored activity to greater than 95%, suggesting that Ca2+ ion is essential to maintain the activity.

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Partial characterization of phosphotriesterase activity from the earthworm, Eisenia andrei

Abstract

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