Various types of microplates made of polystyrene have been widely used for immunoassays. A new microplate suitable for the covalent immobilization of proteins and peptides was developed by thermal deposition of amino-modified parylene (parylene-A) on the microplate. The primary amine groups of the parylene-A was exploited for the covalent coupling of proteins and peptides. The optical transmittance at the wavelength of 400-500 nm was estimated to be suitable for the application to immunoassays. The immobilization efficiency of the parylene-A coated microplate was demonstrated to be far improved in comparison to the conventional microplate by using horseradish peroxidase (HRP), anti-HRP antibody and a peptide with 9-residues as model biomolecules.
Bibliographical noteFunding Information:
This research was supported by the National Research Foundation of Korea(NRF) funded by the Ministry of Education, Science and Technology ( 2009-0073809 , 2009-008-1529 and R15-2004-024-00000-0 ) and by “System IC 2010” project of Ministry of Knowledge Economy and by the Seoul Research and Business Development Program ( 10816 ).
All Science Journal Classification (ASJC) codes
- Immunology and Allergy