Parylene-A coated microplate for covalent immobilization of proteins and peptides

Byung Jin Jeon, Moo Hwan Kim, Jae Chul Pyun

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Various types of microplates made of polystyrene have been widely used for immunoassays. A new microplate suitable for the covalent immobilization of proteins and peptides was developed by thermal deposition of amino-modified parylene (parylene-A) on the microplate. The primary amine groups of the parylene-A was exploited for the covalent coupling of proteins and peptides. The optical transmittance at the wavelength of 400-500 nm was estimated to be suitable for the application to immunoassays. The immobilization efficiency of the parylene-A coated microplate was demonstrated to be far improved in comparison to the conventional microplate by using horseradish peroxidase (HRP), anti-HRP antibody and a peptide with 9-residues as model biomolecules.

Original languageEnglish
Pages (from-to)44-48
Number of pages5
JournalJournal of Immunological Methods
Volume353
Issue number1-2
DOIs
Publication statusPublished - 2010 Feb 1

Fingerprint

Immobilization
Peptides
Horseradish Peroxidase
Immunoassay
Proteins
Polystyrenes
Amines
Hot Temperature
parylene
Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

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Parylene-A coated microplate for covalent immobilization of proteins and peptides. / Jeon, Byung Jin; Kim, Moo Hwan; Pyun, Jae Chul.

In: Journal of Immunological Methods, Vol. 353, No. 1-2, 01.02.2010, p. 44-48.

Research output: Contribution to journalArticle

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