Parylene-matrix chip for small molecule analysis using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Jo Il Kim, Jong Min Park, Min Jung Kang, Jae Chul Pyun

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

RATIONALE In matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS), analyte molecules are known to be ionized by mixing with organic matrix molecules. As the organic matrix molecules are ionized, they generate unreproducible mass peaks such that MALDI-TOF MS is nearly impossible in the low mass-to-charge (m/z) range (<1000). In this work, we aimed to develop a parylene-matrix chip for the detection of small molecules in the low m/z range by using MALDI-TOF MS. METHODS The parylene-matrix chip was fabricated by the deposition of a partially porous parylene-N thin film on a dried organic matrix array. The properties of the parylene thin film were analyzed by atomic force microscopy (AFM) and cyclic voltammetry (CV). Mass spectrometry was performed by using a parylene-matrix chip with eight amino acids as model analytes. RESULTS The surface roughness and the electric conductivity of the parylene-N film were analyzed by AFM and CV analysis to determine its suitability for a parylene-matrix chip. The ionization of samples on the parylene-matrix chip was optimized by adjusting the laser intensity. The feasibility of applying a parylene-matrix chip for small molecule analysis was tested by using eight kinds of amino acids as model analytes and the simultaneous detection of multiple analytes from the amino acid mixture was also demonstrated. CONCLUSIONS The parylene-matrix chip can be applied for the detection of multiple analytes in the m/z ratio range of small molecules (<1000) using MALDI-TOF MS.

Original languageEnglish
Pages (from-to)274-280
Number of pages7
JournalRapid Communications in Mass Spectrometry
Volume28
Issue number3
DOIs
Publication statusPublished - 2014 Feb 15

Fingerprint

Ionization
Mass spectrometry
Desorption
Molecules
Lasers
Amino Acids
Cyclic voltammetry
Atomic force microscopy
parylene
Thin films
Surface roughness

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

Cite this

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title = "Parylene-matrix chip for small molecule analysis using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry",
abstract = "RATIONALE In matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS), analyte molecules are known to be ionized by mixing with organic matrix molecules. As the organic matrix molecules are ionized, they generate unreproducible mass peaks such that MALDI-TOF MS is nearly impossible in the low mass-to-charge (m/z) range (<1000). In this work, we aimed to develop a parylene-matrix chip for the detection of small molecules in the low m/z range by using MALDI-TOF MS. METHODS The parylene-matrix chip was fabricated by the deposition of a partially porous parylene-N thin film on a dried organic matrix array. The properties of the parylene thin film were analyzed by atomic force microscopy (AFM) and cyclic voltammetry (CV). Mass spectrometry was performed by using a parylene-matrix chip with eight amino acids as model analytes. RESULTS The surface roughness and the electric conductivity of the parylene-N film were analyzed by AFM and CV analysis to determine its suitability for a parylene-matrix chip. The ionization of samples on the parylene-matrix chip was optimized by adjusting the laser intensity. The feasibility of applying a parylene-matrix chip for small molecule analysis was tested by using eight kinds of amino acids as model analytes and the simultaneous detection of multiple analytes from the amino acid mixture was also demonstrated. CONCLUSIONS The parylene-matrix chip can be applied for the detection of multiple analytes in the m/z ratio range of small molecules (<1000) using MALDI-TOF MS.",
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Parylene-matrix chip for small molecule analysis using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. / Kim, Jo Il; Park, Jong Min; Kang, Min Jung; Pyun, Jae Chul.

In: Rapid Communications in Mass Spectrometry, Vol. 28, No. 3, 15.02.2014, p. 274-280.

Research output: Contribution to journalArticle

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T1 - Parylene-matrix chip for small molecule analysis using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

AU - Kim, Jo Il

AU - Park, Jong Min

AU - Kang, Min Jung

AU - Pyun, Jae Chul

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N2 - RATIONALE In matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS), analyte molecules are known to be ionized by mixing with organic matrix molecules. As the organic matrix molecules are ionized, they generate unreproducible mass peaks such that MALDI-TOF MS is nearly impossible in the low mass-to-charge (m/z) range (<1000). In this work, we aimed to develop a parylene-matrix chip for the detection of small molecules in the low m/z range by using MALDI-TOF MS. METHODS The parylene-matrix chip was fabricated by the deposition of a partially porous parylene-N thin film on a dried organic matrix array. The properties of the parylene thin film were analyzed by atomic force microscopy (AFM) and cyclic voltammetry (CV). Mass spectrometry was performed by using a parylene-matrix chip with eight amino acids as model analytes. RESULTS The surface roughness and the electric conductivity of the parylene-N film were analyzed by AFM and CV analysis to determine its suitability for a parylene-matrix chip. The ionization of samples on the parylene-matrix chip was optimized by adjusting the laser intensity. The feasibility of applying a parylene-matrix chip for small molecule analysis was tested by using eight kinds of amino acids as model analytes and the simultaneous detection of multiple analytes from the amino acid mixture was also demonstrated. CONCLUSIONS The parylene-matrix chip can be applied for the detection of multiple analytes in the m/z ratio range of small molecules (<1000) using MALDI-TOF MS.

AB - RATIONALE In matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS), analyte molecules are known to be ionized by mixing with organic matrix molecules. As the organic matrix molecules are ionized, they generate unreproducible mass peaks such that MALDI-TOF MS is nearly impossible in the low mass-to-charge (m/z) range (<1000). In this work, we aimed to develop a parylene-matrix chip for the detection of small molecules in the low m/z range by using MALDI-TOF MS. METHODS The parylene-matrix chip was fabricated by the deposition of a partially porous parylene-N thin film on a dried organic matrix array. The properties of the parylene thin film were analyzed by atomic force microscopy (AFM) and cyclic voltammetry (CV). Mass spectrometry was performed by using a parylene-matrix chip with eight amino acids as model analytes. RESULTS The surface roughness and the electric conductivity of the parylene-N film were analyzed by AFM and CV analysis to determine its suitability for a parylene-matrix chip. The ionization of samples on the parylene-matrix chip was optimized by adjusting the laser intensity. The feasibility of applying a parylene-matrix chip for small molecule analysis was tested by using eight kinds of amino acids as model analytes and the simultaneous detection of multiple analytes from the amino acid mixture was also demonstrated. CONCLUSIONS The parylene-matrix chip can be applied for the detection of multiple analytes in the m/z ratio range of small molecules (<1000) using MALDI-TOF MS.

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