Pathogen-induced expression of cyclo-oxygenase homologue in hot pepper (Capsicum annuum cv. Pukang)

Young Cheol Kim, So Young Yi, Hyung Gon Mang, Young Sam Seo, Woo Taek Kim, Doil Choi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The hypersensitive reaction (HR) in plants is typified by a rapid and localized cell death at the site of pathogen infection. To understand better the molecular and cellular defence mechanism controlling HR, hot pepper leaves (Capsicum annuum cv. Pukang) were inoculated with the soybean pustule pathogen Xanthomonas campestris pv. glycine 8ra. By using the DD-PCR technique, a cDNA fragment was identified that exhibited a sequence similarity to the recently identified tobacco pathogen-induced oxygenase (PIOX) with homology to animal cyclo-oxygenase (COX). Subsequently, the full-length cDNA clone, pCa-COX1, encoding the COX homologue from the pathogen-inoculated hot pepper leaf cDNA library was isolated. The deduced amino acid sequence of Ca-COX1 shares 85.8% identity with tobacco PIOX and displays a significant degree of sequence identity (21.7-23.7%) with mammalian COXs. The expression of Ca-COX1 was markedly induced at 4-12 h after pathogen infection, while HR cell death on pepper leaves appeared at approximately 15 h post-inoculation. These results are consistent with the notion that the lipid-derived signalling pathway is involved in the initial response of hot pepper plants to pathogen infection.

Original languageEnglish
Pages (from-to)383-385
Number of pages3
JournalJournal of Experimental Botany
Volume53
Issue number367
DOIs
Publication statusPublished - 2002 Jan 1

Fingerprint

Capsicum
hot peppers
prostaglandin synthase
Prostaglandin-Endoperoxide Synthases
Capsicum annuum
Oxygenases
pathogens
hypersensitive response
Tobacco
Cell Death
Complementary DNA
Infection
Xanthomonas campestris
oxygenases
Xanthomonas campestris pv. glycines
Gene Library
cell death
Soybeans
Glycine
tobacco

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

Kim, Young Cheol ; Yi, So Young ; Mang, Hyung Gon ; Seo, Young Sam ; Kim, Woo Taek ; Choi, Doil. / Pathogen-induced expression of cyclo-oxygenase homologue in hot pepper (Capsicum annuum cv. Pukang). In: Journal of Experimental Botany. 2002 ; Vol. 53, No. 367. pp. 383-385.
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Pathogen-induced expression of cyclo-oxygenase homologue in hot pepper (Capsicum annuum cv. Pukang). / Kim, Young Cheol; Yi, So Young; Mang, Hyung Gon; Seo, Young Sam; Kim, Woo Taek; Choi, Doil.

In: Journal of Experimental Botany, Vol. 53, No. 367, 01.01.2002, p. 383-385.

Research output: Contribution to journalArticle

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