Patterned Nanowire Electrode Array for Direct Extraction of Photosynthetic Electrons from Multiple Living Algal Cells

Lo Hyun Kim; Yong Jae Kim; Hyeonaug Hong; Dasom Yang; Myungjin Han; Gu Yoo; Hyun Woo Song; Youngcheol Chae; Jae Chul Pyun; Arthur R. Grossman; Won Hyoung Ryu

doi:10.1002/adfm.201602171

Patterned Nanowire Electrode Array for Direct Extraction of Photosynthetic Electrons from Multiple Living Algal Cells

Lo Hyun Kim, Yong Jae Kim, Hyeonaug Hong, Dasom Yang, Myungjin Han, Gu Yoo, Hyun Woo Song, Youngcheol Chae, Jae Chul Pyun, Arthur R. Grossman, Won Hyoung Ryu

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

An electrochemically active nanowire system is fabricated using wet-tapped nanosphere lithography and a single photolithography step. The patterned nanowire/nanoelectrode is inserted into live algal cells, enabling the potential harvesting of photosynthetic electrons from multiple cells simultaneously. Light-dependent extraction of electrons from cells is observed; these electrons are derived from the photosynthetic electron transport chain based on a light intensity-dependence of the reaction coupled with the finding that electron extraction is inhibited in the presence of DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea), a reagent that specifically blocks electron flow out of photosystem II. Insertion of nanoelectrodes into multiple algal cells is achieved, and sequential insertion of cells with the nanoelectrode, followed by subsequent removal of the electrode, yields a corresponding increase and then decrease in light-driven currents. Controlling the intensity of the illumination avoids nearly all photodamage and enables direct extraction of more photosynthetic electrons from multiple cells in parallel, which is sustained for an extended period of time.

Original language	English
Pages (from-to)	7679-7689
Number of pages	11
Journal	Advanced Functional Materials
Volume	26
Issue number	42
DOIs	https://doi.org/10.1002/adfm.201602171
Publication status	Published - 2016 Nov 8

Bibliographical note

Funding Information:
L.H.K. and Y.J.K. contributed equally to this work. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2011–0020285) as well as by the Center for Advanced Meta-Materials(CAMM) funded by the Ministry of Science, ICT and Future Planning as Global Frontier Project (CAMM- 2014M3A6B3063716). The authors thank Prof. Yeu-Chun Kim at KAIST for providing algal cells. It was also supported by the US Department of Energy, Basic Energy Science (DE-S000160) to ARG. The authors appreciate the help of Prof. Dongil Lee at Yonsei University for advice about electrochemical analysis.

Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

All Science Journal Classification (ASJC) codes

Chemistry(all)
Materials Science(all)
Condensed Matter Physics

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10.1002/adfm.201602171

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title = "Patterned Nanowire Electrode Array for Direct Extraction of Photosynthetic Electrons from Multiple Living Algal Cells",

abstract = "An electrochemically active nanowire system is fabricated using wet-tapped nanosphere lithography and a single photolithography step. The patterned nanowire/nanoelectrode is inserted into live algal cells, enabling the potential harvesting of photosynthetic electrons from multiple cells simultaneously. Light-dependent extraction of electrons from cells is observed; these electrons are derived from the photosynthetic electron transport chain based on a light intensity-dependence of the reaction coupled with the finding that electron extraction is inhibited in the presence of DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea), a reagent that specifically blocks electron flow out of photosystem II. Insertion of nanoelectrodes into multiple algal cells is achieved, and sequential insertion of cells with the nanoelectrode, followed by subsequent removal of the electrode, yields a corresponding increase and then decrease in light-driven currents. Controlling the intensity of the illumination avoids nearly all photodamage and enables direct extraction of more photosynthetic electrons from multiple cells in parallel, which is sustained for an extended period of time.",

author = "Kim, {Lo Hyun} and Kim, {Yong Jae} and Hyeonaug Hong and Dasom Yang and Myungjin Han and Gu Yoo and Song, {Hyun Woo} and Youngcheol Chae and Pyun, {Jae Chul} and Grossman, {Arthur R.} and Ryu, {Won Hyoung}",

note = "Funding Information: L.H.K. and Y.J.K. contributed equally to this work. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2011–0020285) as well as by the Center for Advanced Meta-Materials(CAMM) funded by the Ministry of Science, ICT and Future Planning as Global Frontier Project (CAMM- 2014M3A6B3063716). The authors thank Prof. Yeu-Chun Kim at KAIST for providing algal cells. It was also supported by the US Department of Energy, Basic Energy Science (DE-S000160) to ARG. The authors appreciate the help of Prof. Dongil Lee at Yonsei University for advice about electrochemical analysis. Publisher Copyright: {\textcopyright} 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2016",

month = nov,

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doi = "10.1002/adfm.201602171",

language = "English",

volume = "26",

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AU - Kim, Lo Hyun

AU - Kim, Yong Jae

AU - Hong, Hyeonaug

AU - Yang, Dasom

AU - Han, Myungjin

AU - Yoo, Gu

AU - Song, Hyun Woo

AU - Chae, Youngcheol

AU - Pyun, Jae Chul

AU - Grossman, Arthur R.

AU - Ryu, Won Hyoung

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PY - 2016/11/8

Y1 - 2016/11/8

N2 - An electrochemically active nanowire system is fabricated using wet-tapped nanosphere lithography and a single photolithography step. The patterned nanowire/nanoelectrode is inserted into live algal cells, enabling the potential harvesting of photosynthetic electrons from multiple cells simultaneously. Light-dependent extraction of electrons from cells is observed; these electrons are derived from the photosynthetic electron transport chain based on a light intensity-dependence of the reaction coupled with the finding that electron extraction is inhibited in the presence of DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea), a reagent that specifically blocks electron flow out of photosystem II. Insertion of nanoelectrodes into multiple algal cells is achieved, and sequential insertion of cells with the nanoelectrode, followed by subsequent removal of the electrode, yields a corresponding increase and then decrease in light-driven currents. Controlling the intensity of the illumination avoids nearly all photodamage and enables direct extraction of more photosynthetic electrons from multiple cells in parallel, which is sustained for an extended period of time.

AB - An electrochemically active nanowire system is fabricated using wet-tapped nanosphere lithography and a single photolithography step. The patterned nanowire/nanoelectrode is inserted into live algal cells, enabling the potential harvesting of photosynthetic electrons from multiple cells simultaneously. Light-dependent extraction of electrons from cells is observed; these electrons are derived from the photosynthetic electron transport chain based on a light intensity-dependence of the reaction coupled with the finding that electron extraction is inhibited in the presence of DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea), a reagent that specifically blocks electron flow out of photosystem II. Insertion of nanoelectrodes into multiple algal cells is achieved, and sequential insertion of cells with the nanoelectrode, followed by subsequent removal of the electrode, yields a corresponding increase and then decrease in light-driven currents. Controlling the intensity of the illumination avoids nearly all photodamage and enables direct extraction of more photosynthetic electrons from multiple cells in parallel, which is sustained for an extended period of time.

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DO - 10.1002/adfm.201602171

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JO - Advanced Functional Materials

JF - Advanced Functional Materials

IS - 42

ER -

Patterned Nanowire Electrode Array for Direct Extraction of Photosynthetic Electrons from Multiple Living Algal Cells

Abstract

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