Patterning of alternating proteins inside a microfluidic channel for enhanced tumor cell isolation

C. A. Launiere, M. M. Gaskill, J. H. Myung, S. Hong, D. T. Eddington

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here we report on a technique to improve the capture rate of an affinity-based microfluidic CTC isolation device by patterning regions of alternating capture proteins. Of the two capture proteins, anti-epithelial cell adhesion molecule (aEpCAM) provides the specificity for CTC capture, while E-selectin endows robustness that increases CTC capture under shear. This patterning technique may also be useful in other biotechnology applications, as it can be used to spatially control the immobilization of any 2 proteins or protein mixtures inside a sealed microfluidic channel.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1227-1229
Number of pages3
Publication statusPublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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  • Cite this

    Launiere, C. A., Gaskill, M. M., Myung, J. H., Hong, S., & Eddington, D. T. (2011). Patterning of alternating proteins inside a microfluidic channel for enhanced tumor cell isolation. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1227-1229). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).