Patterns and biologic features of p53 mutation types in Korean breast cancer patients

Hyung Won Kim, Hak Min Lee, Seung Hyun Hwang, Sung Gwe Ahn, Kyung A. Lee, Joon Jeong

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose: The p53 gene is one of the most frequently mutated genes in breast cancer. We investigated the patterns and biologic features of p53 gene mutation and evaluated their clinical signifi-cance in Korean breast cancer patients. Methods: Patients who underwent p53 gene sequencing were included. Mutational analysis of exon 5 to exon 9 of the p53 gene was carried out using polymerase chain reaction-denaturing high performance liquid chromatography and direct sequencing. Results: A total of 497 patients were eligible for the present study and p53 gene mutations were detected in 71 cases (14.3%). Mutation of p53 was significantly associated with histologic grading (p< 0.001), estrogen receptor and progesterone receptor status (p< 0.001), HER2 status (p< 0.001), Ki-67 (p=0.028), and tumor size (p=0.004). The most frequent location of p53 mutations was exon 7 and missense mutation was the most common type of mutation. Compared with patients without mutation, there was a statistically significant difference in relapse-free survival of patients with p53 gene mutation and missense mutation (p= 0.020, p= 0.006, respectively). Only p53 missense mutation was an independent prognostic factor for relapse-free survival in multivariate analysis, with an adjusted hazard ratio of 2.29 (95% confidence interval, 1.08-4.89, p=0.031). Conclusion: Mutation of the p53 gene was associated with more aggressive clinicopathologic characteristics and p53 missense mutation was an independent negative prognostic factor in Korean breast cancer patients.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Breast Cancer
Volume17
Issue number1
DOIs
Publication statusPublished - 2014 Mar

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p53 Genes
Breast Neoplasms
Mutation
Missense Mutation
Exons
Recurrence
Survival
Progesterone Receptors
Estrogen Receptors
Multivariate Analysis
High Pressure Liquid Chromatography
Confidence Intervals
Polymerase Chain Reaction
Genes
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Kim, Hyung Won ; Lee, Hak Min ; Hwang, Seung Hyun ; Ahn, Sung Gwe ; Lee, Kyung A. ; Jeong, Joon. / Patterns and biologic features of p53 mutation types in Korean breast cancer patients. In: Journal of Breast Cancer. 2014 ; Vol. 17, No. 1. pp. 1-7.
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abstract = "Purpose: The p53 gene is one of the most frequently mutated genes in breast cancer. We investigated the patterns and biologic features of p53 gene mutation and evaluated their clinical signifi-cance in Korean breast cancer patients. Methods: Patients who underwent p53 gene sequencing were included. Mutational analysis of exon 5 to exon 9 of the p53 gene was carried out using polymerase chain reaction-denaturing high performance liquid chromatography and direct sequencing. Results: A total of 497 patients were eligible for the present study and p53 gene mutations were detected in 71 cases (14.3{\%}). Mutation of p53 was significantly associated with histologic grading (p< 0.001), estrogen receptor and progesterone receptor status (p< 0.001), HER2 status (p< 0.001), Ki-67 (p=0.028), and tumor size (p=0.004). The most frequent location of p53 mutations was exon 7 and missense mutation was the most common type of mutation. Compared with patients without mutation, there was a statistically significant difference in relapse-free survival of patients with p53 gene mutation and missense mutation (p= 0.020, p= 0.006, respectively). Only p53 missense mutation was an independent prognostic factor for relapse-free survival in multivariate analysis, with an adjusted hazard ratio of 2.29 (95{\%} confidence interval, 1.08-4.89, p=0.031). Conclusion: Mutation of the p53 gene was associated with more aggressive clinicopathologic characteristics and p53 missense mutation was an independent negative prognostic factor in Korean breast cancer patients.",
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Patterns and biologic features of p53 mutation types in Korean breast cancer patients. / Kim, Hyung Won; Lee, Hak Min; Hwang, Seung Hyun; Ahn, Sung Gwe; Lee, Kyung A.; Jeong, Joon.

In: Journal of Breast Cancer, Vol. 17, No. 1, 03.2014, p. 1-7.

Research output: Contribution to journalArticle

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N2 - Purpose: The p53 gene is one of the most frequently mutated genes in breast cancer. We investigated the patterns and biologic features of p53 gene mutation and evaluated their clinical signifi-cance in Korean breast cancer patients. Methods: Patients who underwent p53 gene sequencing were included. Mutational analysis of exon 5 to exon 9 of the p53 gene was carried out using polymerase chain reaction-denaturing high performance liquid chromatography and direct sequencing. Results: A total of 497 patients were eligible for the present study and p53 gene mutations were detected in 71 cases (14.3%). Mutation of p53 was significantly associated with histologic grading (p< 0.001), estrogen receptor and progesterone receptor status (p< 0.001), HER2 status (p< 0.001), Ki-67 (p=0.028), and tumor size (p=0.004). The most frequent location of p53 mutations was exon 7 and missense mutation was the most common type of mutation. Compared with patients without mutation, there was a statistically significant difference in relapse-free survival of patients with p53 gene mutation and missense mutation (p= 0.020, p= 0.006, respectively). Only p53 missense mutation was an independent prognostic factor for relapse-free survival in multivariate analysis, with an adjusted hazard ratio of 2.29 (95% confidence interval, 1.08-4.89, p=0.031). Conclusion: Mutation of the p53 gene was associated with more aggressive clinicopathologic characteristics and p53 missense mutation was an independent negative prognostic factor in Korean breast cancer patients.

AB - Purpose: The p53 gene is one of the most frequently mutated genes in breast cancer. We investigated the patterns and biologic features of p53 gene mutation and evaluated their clinical signifi-cance in Korean breast cancer patients. Methods: Patients who underwent p53 gene sequencing were included. Mutational analysis of exon 5 to exon 9 of the p53 gene was carried out using polymerase chain reaction-denaturing high performance liquid chromatography and direct sequencing. Results: A total of 497 patients were eligible for the present study and p53 gene mutations were detected in 71 cases (14.3%). Mutation of p53 was significantly associated with histologic grading (p< 0.001), estrogen receptor and progesterone receptor status (p< 0.001), HER2 status (p< 0.001), Ki-67 (p=0.028), and tumor size (p=0.004). The most frequent location of p53 mutations was exon 7 and missense mutation was the most common type of mutation. Compared with patients without mutation, there was a statistically significant difference in relapse-free survival of patients with p53 gene mutation and missense mutation (p= 0.020, p= 0.006, respectively). Only p53 missense mutation was an independent prognostic factor for relapse-free survival in multivariate analysis, with an adjusted hazard ratio of 2.29 (95% confidence interval, 1.08-4.89, p=0.031). Conclusion: Mutation of the p53 gene was associated with more aggressive clinicopathologic characteristics and p53 missense mutation was an independent negative prognostic factor in Korean breast cancer patients.

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