Patterns of sequence conservation at termini of long terminal repeat (LRT) retrotransposons and DNA transposons in the human genome

Lessons from phage Mu

In suk Lee, Rasika M. Harshley

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Long terminal repeat (LTR) retrotransposons and DNA transposons are transposable elements (TEs) that perform cleavage and transfer at precise DNA positions. Here, we present statistical analyses of sequences found at the termini of precise TEs in the human genome. The results show that the terminal di- and trinucleotides of these TEs are highly conserved. 5′TG...CA3′ occurs most frequently at the termini of LTR retrotransposons, while 5′CAG...CTG3′ occurs most frequently in DNA transposons. Interestingly, these sequences are the most flexible base pair steps in DNA. Both the sequence preference and the degree of conservation of each position within the human LTR dinucleotide termini are remarkably similar to those experimentally demonstrated in transposable phage Mu. We discuss the significance of these observations and their implication for the function of terminal residues in the transposition of precise TEs.

Original languageEnglish
Pages (from-to)4531-4540
Number of pages10
JournalNucleic acids research
Volume31
Issue number15
DOIs
Publication statusPublished - 2003 Aug 1

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Bacteriophage mu
Retroelements
DNA Transposable Elements
Terminal Repeat Sequences
Human Genome
DNA
Base Pairing
Bacteriophages
Sequence Analysis

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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