Pax6 expressed in osteocytes inhibits canonical Wnt signaling

Ajita Jami, Jogeswar Gadi, Min Jung Lee, Eun Jin Kim, Mi Jeong Lee, Hansung Jung, Hong Hee Kim, Sungkil Lim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The transcription factor Pax6, which belongs to the paired box-containing gene family, regulates developmental processes, especially in the eyes, central nervous tissues and craniofacial structures. However, the role of Pax6 in bone has never been studied exclusively. Here we report that Pax6 is expressed at both the mRNA and protein level in the calvaria and long bones of adult mice as well as osteocyte-like MLOY4 cells and suppresses the canonical Wnt signaling pathway. Moreover, the expression levels of Pax6 were much higher in the calvaria than the long bones, and Pax6 was also expressed at E16 to E18 in both the calvaria and long bones. Knockdown of Pax6 in MLOY4 cells did not affect cell proliferation or survival; however, the expression of Sost, an osteocyte marker gene, was significantly decreased. In addition, the overexpression of Pax6 suppressed the canonical Wnt signaling pathway by enhancing the expression of Sost. Furthermore, we also demonstrated that Pax6 binds to the Sost promoter and that stimulation of Sost transcription by Pax6 was dependent on a specific Pax6-binding sequence within the promoter. In conclusion, the results of the present study suggest that Pax6 is expressed in bone and may play an important role in osteocyte differentiation by controlling canonical Wnt signaling.

Original languageEnglish
Pages (from-to)305-312
Number of pages8
JournalMolecules and cells
Volume35
Issue number4
DOIs
Publication statusPublished - 2013 Apr 1

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Osteocytes
Wnt Signaling Pathway
Bone and Bones
Skull
Nerve Tissue
Genes
Cell Survival
Transcription Factors
Cell Proliferation
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Jami, Ajita ; Gadi, Jogeswar ; Lee, Min Jung ; Kim, Eun Jin ; Lee, Mi Jeong ; Jung, Hansung ; Kim, Hong Hee ; Lim, Sungkil. / Pax6 expressed in osteocytes inhibits canonical Wnt signaling. In: Molecules and cells. 2013 ; Vol. 35, No. 4. pp. 305-312.
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abstract = "The transcription factor Pax6, which belongs to the paired box-containing gene family, regulates developmental processes, especially in the eyes, central nervous tissues and craniofacial structures. However, the role of Pax6 in bone has never been studied exclusively. Here we report that Pax6 is expressed at both the mRNA and protein level in the calvaria and long bones of adult mice as well as osteocyte-like MLOY4 cells and suppresses the canonical Wnt signaling pathway. Moreover, the expression levels of Pax6 were much higher in the calvaria than the long bones, and Pax6 was also expressed at E16 to E18 in both the calvaria and long bones. Knockdown of Pax6 in MLOY4 cells did not affect cell proliferation or survival; however, the expression of Sost, an osteocyte marker gene, was significantly decreased. In addition, the overexpression of Pax6 suppressed the canonical Wnt signaling pathway by enhancing the expression of Sost. Furthermore, we also demonstrated that Pax6 binds to the Sost promoter and that stimulation of Sost transcription by Pax6 was dependent on a specific Pax6-binding sequence within the promoter. In conclusion, the results of the present study suggest that Pax6 is expressed in bone and may play an important role in osteocyte differentiation by controlling canonical Wnt signaling.",
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Jami, A, Gadi, J, Lee, MJ, Kim, EJ, Lee, MJ, Jung, H, Kim, HH & Lim, S 2013, 'Pax6 expressed in osteocytes inhibits canonical Wnt signaling', Molecules and cells, vol. 35, no. 4, pp. 305-312. https://doi.org/10.1007/s10059-013-2310-0

Pax6 expressed in osteocytes inhibits canonical Wnt signaling. / Jami, Ajita; Gadi, Jogeswar; Lee, Min Jung; Kim, Eun Jin; Lee, Mi Jeong; Jung, Hansung; Kim, Hong Hee; Lim, Sungkil.

In: Molecules and cells, Vol. 35, No. 4, 01.04.2013, p. 305-312.

Research output: Contribution to journalArticle

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AU - Jami, Ajita

AU - Gadi, Jogeswar

AU - Lee, Min Jung

AU - Kim, Eun Jin

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AU - Jung, Hansung

AU - Kim, Hong Hee

AU - Lim, Sungkil

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