PCR-reverse blot hybridization assay for fast and accurate identification of causative species in superficial fungal infections

S. Y. Park, B. K. Kim, H. Y. Wang, S. H. Kim, H. J. Kim, H. Y. Lee, Eung Ho Choi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background Superficial fungal infections are a very common problem in dermatological clinics. The diagnostic method of fungal culture is time-consuming and has inconsistent sensitivity. Therefore, a practical method for rapid and accurate identification of the species causing superficial fungal infections is needed. Aim To compare PCR-reverse blot hybridization assay (PCR-REBA) with conventional fungal diagnostic methods so as to determine the reliability of PCR-REBA for the diagnosis and species identification in superficial fungal infections. Methods Potassium hydroxide (KOH) preparation, fungal culture, conventional real-time PCR and PCR-REBA were used to assess 83 specimens, and the results from each method were compared. Results Of the 83 specimens, 44 specimens that were positive by fungal culture had 62.7% agreement with PCR-REBA. Compared with real-time PCR, there was 68.7% agreement with fungal culture, but 91.6% agreement with PCR-REBA. When the comparison was made using the 55 specimens that gave positive results in both KOH preparation and fungal culture, there was 85.5% agreement with real-time PCR for fungal culture, but 94.5% agreement with PCR-REBA. Conclusions Compared with KOH preparation or fungal culture, PCR-REBA has higher sensitivity and specificity. Therefore, PCR-REBA could be a useful method in clinical settings because it can identify species quickly and accurately, and can also determine the existence of pathogens.

Original languageEnglish
Pages (from-to)359-365
Number of pages7
JournalClinical and Experimental Dermatology
Volume41
Issue number4
DOIs
Publication statusPublished - 2016 Jun 1

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Mycoses
Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Park, S. Y. ; Kim, B. K. ; Wang, H. Y. ; Kim, S. H. ; Kim, H. J. ; Lee, H. Y. ; Choi, Eung Ho. / PCR-reverse blot hybridization assay for fast and accurate identification of causative species in superficial fungal infections. In: Clinical and Experimental Dermatology. 2016 ; Vol. 41, No. 4. pp. 359-365.
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abstract = "Background Superficial fungal infections are a very common problem in dermatological clinics. The diagnostic method of fungal culture is time-consuming and has inconsistent sensitivity. Therefore, a practical method for rapid and accurate identification of the species causing superficial fungal infections is needed. Aim To compare PCR-reverse blot hybridization assay (PCR-REBA) with conventional fungal diagnostic methods so as to determine the reliability of PCR-REBA for the diagnosis and species identification in superficial fungal infections. Methods Potassium hydroxide (KOH) preparation, fungal culture, conventional real-time PCR and PCR-REBA were used to assess 83 specimens, and the results from each method were compared. Results Of the 83 specimens, 44 specimens that were positive by fungal culture had 62.7{\%} agreement with PCR-REBA. Compared with real-time PCR, there was 68.7{\%} agreement with fungal culture, but 91.6{\%} agreement with PCR-REBA. When the comparison was made using the 55 specimens that gave positive results in both KOH preparation and fungal culture, there was 85.5{\%} agreement with real-time PCR for fungal culture, but 94.5{\%} agreement with PCR-REBA. Conclusions Compared with KOH preparation or fungal culture, PCR-REBA has higher sensitivity and specificity. Therefore, PCR-REBA could be a useful method in clinical settings because it can identify species quickly and accurately, and can also determine the existence of pathogens.",
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PCR-reverse blot hybridization assay for fast and accurate identification of causative species in superficial fungal infections. / Park, S. Y.; Kim, B. K.; Wang, H. Y.; Kim, S. H.; Kim, H. J.; Lee, H. Y.; Choi, Eung Ho.

In: Clinical and Experimental Dermatology, Vol. 41, No. 4, 01.06.2016, p. 359-365.

Research output: Contribution to journalArticle

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N2 - Background Superficial fungal infections are a very common problem in dermatological clinics. The diagnostic method of fungal culture is time-consuming and has inconsistent sensitivity. Therefore, a practical method for rapid and accurate identification of the species causing superficial fungal infections is needed. Aim To compare PCR-reverse blot hybridization assay (PCR-REBA) with conventional fungal diagnostic methods so as to determine the reliability of PCR-REBA for the diagnosis and species identification in superficial fungal infections. Methods Potassium hydroxide (KOH) preparation, fungal culture, conventional real-time PCR and PCR-REBA were used to assess 83 specimens, and the results from each method were compared. Results Of the 83 specimens, 44 specimens that were positive by fungal culture had 62.7% agreement with PCR-REBA. Compared with real-time PCR, there was 68.7% agreement with fungal culture, but 91.6% agreement with PCR-REBA. When the comparison was made using the 55 specimens that gave positive results in both KOH preparation and fungal culture, there was 85.5% agreement with real-time PCR for fungal culture, but 94.5% agreement with PCR-REBA. Conclusions Compared with KOH preparation or fungal culture, PCR-REBA has higher sensitivity and specificity. Therefore, PCR-REBA could be a useful method in clinical settings because it can identify species quickly and accurately, and can also determine the existence of pathogens.

AB - Background Superficial fungal infections are a very common problem in dermatological clinics. The diagnostic method of fungal culture is time-consuming and has inconsistent sensitivity. Therefore, a practical method for rapid and accurate identification of the species causing superficial fungal infections is needed. Aim To compare PCR-reverse blot hybridization assay (PCR-REBA) with conventional fungal diagnostic methods so as to determine the reliability of PCR-REBA for the diagnosis and species identification in superficial fungal infections. Methods Potassium hydroxide (KOH) preparation, fungal culture, conventional real-time PCR and PCR-REBA were used to assess 83 specimens, and the results from each method were compared. Results Of the 83 specimens, 44 specimens that were positive by fungal culture had 62.7% agreement with PCR-REBA. Compared with real-time PCR, there was 68.7% agreement with fungal culture, but 91.6% agreement with PCR-REBA. When the comparison was made using the 55 specimens that gave positive results in both KOH preparation and fungal culture, there was 85.5% agreement with real-time PCR for fungal culture, but 94.5% agreement with PCR-REBA. Conclusions Compared with KOH preparation or fungal culture, PCR-REBA has higher sensitivity and specificity. Therefore, PCR-REBA could be a useful method in clinical settings because it can identify species quickly and accurately, and can also determine the existence of pathogens.

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